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Regulation of Androgen and Vitamin D Receptors by 1,25-Dihydroxyvitamin D 3 in Human Prostate Epithelial and Stromal Cells

2003; Lippincott Williams & Wilkins; Volume: 170; Issue: 1 Linguagem: Inglês

10.1097/01.ju.0000060117.61770.35

1527-3792

Eddy S. Leman, Fernando DeMiguel, Allen C. Gao, Robert H. Getzenberg,

Vitamin D Research Studies

No AccessJournal of UrologyINVESTIGATIVE UROLOGY1 Jul 2003Regulation of Androgen and Vitamin D Receptors by 1,25-Dihydroxyvitamin D3 in Human Prostate Epithelial and Stromal Cells EDDY S. LEMAN, FERNANDO DeMIGUEL, ALLEN C. GAO, and ROBERT H. GETZENBERG EDDY S. LEMANEDDY S. LEMAN More articles by this author , FERNANDO DeMIGUELFERNANDO DeMIGUEL More articles by this author , ALLEN C. GAOALLEN C. GAO More articles by this author , and ROBERT H. GETZENBERGROBERT H. GETZENBERG More articles by this author View All Author Informationhttps://doi.org/10.1097/01.ju.0000060117.61770.35AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: The mechanisms of the interaction between 1,25-dihydroxyvitamin D3 (1,25 D) and androgens, and their respective receptors in their action on the prostate are not completely understood. We examined the interplay of 1,25 D and androgens on the epithelial and stromal cells of the prostate. Materials and Methods: The human neonatal prostatic epithelial cell line 267B-1 (BRFF, Inc., Ijamsville, Maryland) and primary cultures of human prostate stromal cells were treated with medium containing 5 or 10 μM 1,25 D or ethanol (control) in the presence or absence of 10 nM dihydrotestosterone (DHT) (Sigma Chemical Co., St. Louis, Missouri). Protein levels of androgen receptor (AR) and vitamin D receptor (VDR) were determined by immunoblot analysis of whole cell extracts. Electrophoresis mobility shift assays were used to determine AR and VDR DNA binding activities. Results: The VDR protein level of 267B-1 cells was increased in the presence of 1,25 D (with the maximum effects seen at 24 hours) regardless of the presence or absence of DHT. In addition, exogenous DHT increased the AR and VDR DNA binding activities of 267B-1 and stromal cells in the presence of 1,25 D. Conclusions: ARs in the normal prostate are regulated by androgens, whereas VDRs in the normal prostate can be regulated by 1,25 D as well as by other androgens such as testosterone. This finding further supports the concept that 1,25 D as a steroid hormone, in addition to other androgens such as DHT, may have a role in the growth and differentiation of normal prostate. References 1 : Structure-function relationships in the vitamin D endocrine system. Endocr Rev1995; 16: 200. Google Scholar 2 : The human prostatic carcinoma cell line LNCaP expresses biologically active, specific receptors for 1 alpha, 25-dihydroxyvitamin D3. Cancer Res1992; 52: 515. Medline, Google Scholar 3 : Antiproliferative effects of 1, 25-dihydroxyvitamin D3 on primary cultures of human prostatic cells. Cancer Res1994; 54: 805. Google Scholar 4 : 1, 25-Dihydroxy-16-ene-23-yne-vitamin D3 and prostate cancer cell proliferation in vivo. Urology1995; 46: 365. 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Google Scholar From the Department of Urology (ESL, FD, ACG, RHG), Cellular and Molecular Pathology Training Program (ESL, ACG, RHG) and University of Pittsburgh Cancer Institute (ESL, ACG, RHG), University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania© 2003 by American Urological Association, Inc.FiguresReferencesRelatedDetailsCited byNangia A, Hill O, Waterman M, Schwender C and Memoli V (2007) Testicular Maturation Arrest to Testis Cancer: Spectrum of Expression of the Vitamin D Receptor and Vitamin D Treatment In VitroJournal of Urology, VOL. 178, NO. 3, (1092-1096), Online publication date: 1-Sep-2007. Volume 170Issue 1July 2003Page: 235-240 Advertisement Copyright & Permissions© 2003 by American Urological Association, Inc.Keywordsreceptors, androgenssteroidscalcitriolandrogensprostateMetricsAuthor Information EDDY S. LEMAN More articles by this author FERNANDO DeMIGUEL More articles by this author ALLEN C. GAO More articles by this author ROBERT H. 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