Portal de Periódicos da CAPES

Epstein-Barr virus-induced proteins II. Analysis of surface polypeptides from EBV-producing and -superinfected cells by immunoprecipitation

1980; Elsevier BV; Volume: 102; Issue: 2 Linguagem: Inglês

10.1016/0042-6822(80)90107-5

1096-0341

Nikolaus Mueller‐Lantzsch, Brigitte Georg, Naoki Yamamoto, Harald zur Hausen,

Herpesvirus Infections and Treatments

Cells of the Epstein-Barr virus (EBV)-producing line P3HR-1 induced by the tumor promoter TPA and NC37 cells Superinfected with P3HR-1 EBV were surface labeled with 125I by the lactoperoxidase method and analyzed by immunoprecipitation with human VCA+ MA+ sera for virus-induced cell surface polypeptides. Two dominant polypeptides with molecular weights of 80,000 and 250,000 were specifically precipitated. In addition, only traces of polypeptides with 130,000 and 140,000 MW were identified on P3HR-1-EBV-producing cells. The surface of superinfected NC37 cells contained two polypeptides of 80,000 and 140,000 molecular weight. Our experiments demonstrated that the synthesis of the 140,000 polypeptide is Ara C sensitive, while the 80,000 polypeptide is Ara-C insensitive. Both polypeptides were found to be identical in size with [35S]-methionine- and 125I-labeled 80,000 and 140,000 envelope polypeptides from purified virus particles. These results may indicate that the identified polypeptides carry antigenic determinants of the EBV-induced membrane antigen (MA) complex.