Portal de Periódicos da CAPES

Detection of Zearalenone and Its Metabolites in Naturally Contaminated Porcine Follicular Fluid by Using Liquid Chromatography-Tandem Mass Spectrometry

2010; Japanese Society of Animal Reproduction; Volume: 57; Issue: 2 Linguagem: Inglês

10.1262/jrd.10-106m

1348-4400

Rentsenkhand Sambuu, Mitsuhiro Takagi, S. Shiga, Seiichi Uno, Emiko Kokushi, Zhao Namula, Takeshige Otoi, Akio Miyamoto, Eisaburo DEGUCHI, Johanna Fink‐Gremmels,

Plant Disease Resistance and Genetics

Zearalenone (ZEN) and its metabolites are important nonsteroidal estrogenic mycotoxins that cause reproductive disorders in domestic animals, especially pigs. We aimed to simultaneously detect ZEN and its metabolites á-zearalenol (α-ZOL) and β-zearalenol (β-ZOL) in porcine follicular fluid (FF) by liquid chromatography-tandem mass spectrometry. ZEN and α-ZOL, but not β-ZOL, were detected in all pooled FF samples collected from coexisting follicles (diameter ≥ 6 mm) within 10 ovaries. Furthermore, ZEN and α-ZOL were detected in samples pretreated with β-glucuronidase/arylsulfatase, but not in those left untreated, suggesting that the FF samples contained glucuronide-conjugated forms of the mycotoxins that may be less harmful to porcine oocytes due to glucuronidation affecting the receptor binding. Nonetheless, the effects of the glucuronide-conjugated forms should be studied, both in vitro and in vivo.