Isolation, ultrastructure and chemical composition of the outermost layer (“exo-layer”) of the Epidermophyton floccosum cell wall
1975; Elsevier BV; Volume: 394; Issue: 4 Linguagem: Inglês
10.1016/0005-2736(75)90141-8
ISSN1879-2642
Autores Tópico(s)Botanical Research and Applications
ResumoThe outer-most layer ("exo-layer") of the wall was isolated from cell walls of Epidermophyton floccosum. The pure cell walls, obtained by disruption in a Ribi cell fractionator, sonication and centrifugation, were digested with snail enzyme for 12 h. Thereafter, the exo-layer preparation was obtained as the fraction resistant to the snail enzyme. Electron microscopy showed that the exo-layer is a thin, stranded network structure 10-20 nm thick. Chemical analysis of the exo-layer showed that the main components are protein (63 percent), mannose (10 percent) and glucosamine (17 percent). Sodium dodecyl sulfate polyacrylamide gel electrophoresis has revealed that the main band is a glycoprotein containing mannose.
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