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Evidence against a role of cyclic nucleotides in the regulation of anaphylactic histamine release in isolated rat mast cells

1981; Wiley; Volume: 112; Issue: 1 Linguagem: Inglês

10.1111/j.1748-1716.1981.tb06781.x

1365-201X

A. Sydbom, Bertil B. Fredholm, Börje Uvnäs,

Coagulation, Bradykinin, Polyphosphates, and Angioedema

The effect of different phosphodiesterase (PDE) inhibitors on the antigen or 48/80 induced histamine release from isolated Hooded Lister rat mast cells was tested. The unselective PDE inhibitors theophylline (2.5 mM) and IBMX (0.2 mM) and the selective cyclic GMP PDE inhibitor M & B 22948 (0.1 mM) inhibited the antigen induced histamine release by 50% while 48/80 induced release was inhibited by about 25%. The cyclic AMP selective PDE inhibitors ICI 63197 (0.5 mM) or Ro 20–1724 (0.2 mM) had no effect on 48/80 induced histamine release but tended to enhance antigen induced release. There was no correlation between the measured levels of cyclic AMP and the effect on histamine release by the