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High-performance liquid chromatographic detector based on near-infrared semiconductor laser fluorimetry

1986; Elsevier BV; Volume: 187; Linguagem: Inglês

10.1016/s0003-2670(00)82931-1

1873-4324

Kouji Sauda, Totaro Imasaka, Nobuhiko Ishibashi,

Spectroscopy and Laser Applications

Three polymethine dyes, which fluoresce at 780–820 nm, are separated by reversed-phase chromatography and detected fluorimetrically with a semiconductor laser for excitation. The detection limit for anhydro-3,3,3′,3′-tetramethyl-1,1′-bis(4-sulfomethyl)-4,5,4′,5′-dibenzoindotricarbocyanine hydroxide (sodium salt) is 0.3 pg, which is almost three orders of magnitude better than the value obtained by conventional fluorimetry.