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Flow Microcalorimetry Applied to the Enzymatic Determination of Urea

1983; Taylor & Francis; Volume: 16; Issue: 1 Linguagem: Inglês

10.1080/00032718308064456

1532-236X

V. Carunchio, Manila Antonelli, Romano Bucci, Andrea Magrì,

Enzyme Catalysis and Immobilization

Abstract A flow microcalorimeter in the mixing mode has been employed to verify the possible application of calorimetric techniques in studying enzymatic reactions. In order to illustrate the optimum operative conditions, with special regard to chemical and instrumental factors affecting the reaction heat quantity, the urea enzymatic decomposition, which is a well studied reaction, was chosen.