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Structured illumination microscopy of autofluorescent aggregations in human tissue

2010; Elsevier BV; Volume: 42; Issue: 4 Linguagem: Inglês

10.1016/j.micron.2010.06.016

1878-4291

Gerrit Best, Roman Amberger, David Baddeley, Thomas Ach, Stefan Dithmar, Rainer Heintzmann, Christoph Cremer,

Optical Imaging and Spectroscopy Techniques

Sections from human eye tissue were analyzed with Structured Illumination Microscopy (SIM) using a specially designed microscope setup. In this microscope the structured illumination was generated with a Twyman-Green Interferometer. This SIM technique allowed us to acquire light-optical images of autofluorophore distributions in the tissue with previously unmatched optical resolution. In this work the unique setup of the microscope made possible the application of SIM with three different excitation wavelengths (488, 568 and 647 nm), thus enabling us to gather spectral information about the autofluorescence signal.