Genetic analysis of adenovirus type 2 VI. A temperature-sensitive mutant defective for DNA encapsidation
1977; Elsevier BV; Volume: 81; Issue: 1 Linguagem: Inglês
10.1016/0042-6822(77)90064-2
ISSN1096-0341
Autores Tópico(s)Viral Infectious Diseases and Gene Expression in Insects
ResumoH2ts4 is a mutant defective in a late function. Centrifugation experiments in CsCl showed that at the nonpermissive temperature (39°) the mutant could produce only top components (TC). When examined in an electron microscope these TC appeared to be morphologically normal. Pulse-chase experiments revealed that proteins synthesized at 39° failed to assemble efficiently into virions when a shift-down to 33° was done immediately after the pulse. DNA encapsidation was completely inhibited when a chase was done at 39° prior to the shift-down to 33°. When [35S]methionine-labeled polypeptides synthesized by infected cells were analyzed on sodium dodecyl sulfate-polyacrylamide gels it was found that at 39° ts4 was defective in the processing of PVII. The postcleavage polypeptides VII, VIII, X, XI, and XII were virtually absent. Temperature-shift experiments showed that processing was temperature sensitive in the shift-up experiments only. Electrophoresis of purified TC grown at 39° showed that the TC of ts4 differ from those of the wild type (wt) only in the slightly higher levels of polypeptides VIII, XI, and XII in the ts4-TC. Polypeptide X was absent from both wt and ts4-TC.
Referência(s)