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Use of a laser to detect viable but immotile spermatozoa

2004; Wiley; Volume: 36; Issue: 6 Linguagem: Inglês

10.1111/j.1439-0272.2004.00636.x

1439-0272

Tahsin Murad Aktan, Markus Montag, Selçuk Duman, Hüseyin Görkemli, Klaus Rink, Talat Yurdakul,

Reproductive Health and Technologies

AndrologiaVolume 36, Issue 6 p. 366-369 Use of a laser to detect viable but immotile spermatozoa T. M. Aktan, T. M. Aktan Department of Histology and Embryology, Meram Medical Faculty, Selcuk University, Konya, TurkeySearch for more papers by this authorM. Montag, M. Montag Department of Gynecological Endocrinology and Reproductive Medicine, Women's Hospital, University of Bonn, GermanySearch for more papers by this authorS. Duman, S. Duman Department of Histology and Embryology, Meram Medical Faculty, Selcuk University, Konya, TurkeySearch for more papers by this authorH. Gorkemli, H. Gorkemli Department of Obstetrics and Gynecology, Meram Medical Faculty, Selcuk University, Konya, TurkeySearch for more papers by this authorK. Rink, K. Rink Laboratory of Applied Optics, Federal Institute of Technology, Lausanne, SwitzerlandSearch for more papers by this authorT. Yurdakul, T. Yurdakul Department of Urology, Meram Medical Faculty, Selcuk University, Konya, TurkeySearch for more papers by this author T. M. Aktan, T. M. Aktan Department of Histology and Embryology, Meram Medical Faculty, Selcuk University, Konya, TurkeySearch for more papers by this authorM. Montag, M. Montag Department of Gynecological Endocrinology and Reproductive Medicine, Women's Hospital, University of Bonn, GermanySearch for more papers by this authorS. Duman, S. Duman Department of Histology and Embryology, Meram Medical Faculty, Selcuk University, Konya, TurkeySearch for more papers by this authorH. Gorkemli, H. Gorkemli Department of Obstetrics and Gynecology, Meram Medical Faculty, Selcuk University, Konya, TurkeySearch for more papers by this authorK. Rink, K. Rink Laboratory of Applied Optics, Federal Institute of Technology, Lausanne, SwitzerlandSearch for more papers by this authorT. Yurdakul, T. Yurdakul Department of Urology, Meram Medical Faculty, Selcuk University, Konya, TurkeySearch for more papers by this author First published: 15 November 2004 https://doi.org/10.1111/j.1439-0272.2004.00636.xCitations: 70 T. Murad Aktan, PhD, Department of Histology and Embryology, Selcuk University, Meram Medical Faculty, Beysehir Yolu Akyokus, 42080 Konya, Turkey. Tel.: +90 332 223 6731; Fax: +90 332 323 9930; E-mail: [email protected] Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Abstract Summary. A prerequisite for the success of intracytoplasmic sperm injection (ICSI) is the use of viable sperm that will support oocyte activation and embryo development. Usually, spermatozoa are selected according to motility, which is a clear indication for viability. However, in cases with complete asthenozoospermia, it is impossible to select viable sperm by morphological means. Here we report for the first time on the use of a laser to discriminate between viable and dead immotile spermatozoa. The application of a single laser shot to the far end of the sperm tail causes a curling of the sperm tail only in viable sperm, similar to the reaction observed in the hypo-osmotic swelling test. Using this method for sperm selection prior to ICSI we achieved high fertilization and cleavage rates in cases with immotile sperm in fresh testicular biopsy material as well in cases with ejaculated immotile sperm. 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