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An Antiviral Agent (46NW-04A) Produced by Pseudomonas sp. and Its Activity against Fish Viruses

1990; Wiley; Volume: 2; Issue: 1 Linguagem: Inglês

10.1577/1548-8667(1990)002 2.3.co;2

1548-8667

Takahisa Kimura, Mamoru Yoshimizu, Yoshio Ezura, Yuto Kamei,

Microbial infections and disease research

Journal of Aquatic Animal HealthVolume 2, Issue 1 p. 12-20 Article An Antiviral Agent (46NW-04A) Produced by Pseudomonas sp. and Its Activity against Fish Viruses Takahisa Kimura, Takahisa Kimura Laboratory for Microbiology, Faculty of Fisheries Hokkaido University, Hakodate, 041 JapanSearch for more papers by this authorMamoru Yoshimizu, Mamoru Yoshimizu Laboratory for Microbiology, Faculty of Fisheries Hokkaido University, Hakodate, 041 JapanSearch for more papers by this authorYoshio Ezura, Yoshio Ezura Laboratory for Microbiology, Faculty of Fisheries Hokkaido University, Hakodate, 041 JapanSearch for more papers by this authorYuto Kamei, Yuto Kamei Laboratory for Microbiology, Faculty of Fisheries Hokkaido University, Hakodate, 041 JapanSearch for more papers by this author Takahisa Kimura, Takahisa Kimura Laboratory for Microbiology, Faculty of Fisheries Hokkaido University, Hakodate, 041 JapanSearch for more papers by this authorMamoru Yoshimizu, Mamoru Yoshimizu Laboratory for Microbiology, Faculty of Fisheries Hokkaido University, Hakodate, 041 JapanSearch for more papers by this authorYoshio Ezura, Yoshio Ezura Laboratory for Microbiology, Faculty of Fisheries Hokkaido University, Hakodate, 041 JapanSearch for more papers by this authorYuto Kamei, Yuto Kamei Laboratory for Microbiology, Faculty of Fisheries Hokkaido University, Hakodate, 041 JapanSearch for more papers by this author First published: 1 January 1990 https://doi.org/10.1577/1548-8667(1990)002 2.3.CO;2Citations: 13AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Abstract The antiviral agent 46NW-04A was isolated and characterized from cell-free culture fluid of Pseudomonas sp. 46NW-04 isolated from the aquatic environment. Production of the antiviral substance was maximal at 25°C during days 2–3 of bacterial incubation. Extraction from 30 L of culture fluid by ethyl acetate and purification by thin-layer chromatography on silica gel resulted in 709 mg of the purified antiviral material. Molecular weight of this substance was 1,126 by secondary ionization mass spectrometry, and chemical properties suggested that 46NW-04A was a peptide. Its antiviral activity, measured as the concentration causing 100% plaque reduction, was 25 μg/mL against Oncorhynchus masou virus and infectious hematopoietic necrosis virus. However, no antiviral activity was observed against infectious pancreatic necrosis virus at the concentrations tested. Pseudomonas sp. 46NW-04 was identified as Pseudomonas fluorescens biovar I. Citing Literature Volume2, Issue11 January 1990Pages 12-20 RelatedInformation