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Vaccinia virus serves as an efficient vector for expressing heterologous proteins in human NTera 2 neurons

1996; Wiley; Volume: 374; Issue: 4 Linguagem: Inglês

10.1002/(sici)1096-9861(19961028)374

1096-9861

David G. Cook, Raymond Scott Turner, Dennis L. Kolson, V.M.-Y. Lee, Robert W. Doms,

Herpesvirus Infections and Treatments

Journal of Comparative NeurologyVolume 374, Issue 4 p. 481-492 Vaccinia virus serves as an efficient vector for expressing heterologous proteins in human NTera 2 neurons David G. Cook, David G. Cook Departments of Pathology and Laboratory Medicine and Neurology University of Pennsylvania, Philadelphia, Pennsylvania 19104Search for more papers by this authorR. Scott Turner, R. Scott Turner VAMC GRECC and Department of Neurology, University of Michigan, Ann Arbor, Michigan 48105Search for more papers by this authorDennis L. Kolson, Dennis L. Kolson Departments of Pathology and Laboratory Medicine and Neurology University of Pennsylvania, Philadelphia, Pennsylvania 19104Search for more papers by this authorVirginia M.-Y. Lee, Virginia M.-Y. Lee Departments of Pathology and Laboratory Medicine and Neurology University of Pennsylvania, Philadelphia, Pennsylvania 19104Search for more papers by this authorRobert W. Doms, Corresponding Author Robert W. Doms Departments of Pathology and Laboratory Medicine and Neurology University of Pennsylvania, Philadelphia, Pennsylvania 19104Department of Pathology and Laboratory Medicine, University of Pennsylvania, SCL-1, Rm. 512, 422 Curie Blvd., Philadelphia, PA 19104Search for more papers by this author David G. Cook, David G. Cook Departments of Pathology and Laboratory Medicine and Neurology University of Pennsylvania, Philadelphia, Pennsylvania 19104Search for more papers by this authorR. Scott Turner, R. Scott Turner VAMC GRECC and Department of Neurology, University of Michigan, Ann Arbor, Michigan 48105Search for more papers by this authorDennis L. Kolson, Dennis L. Kolson Departments of Pathology and Laboratory Medicine and Neurology University of Pennsylvania, Philadelphia, Pennsylvania 19104Search for more papers by this authorVirginia M.-Y. Lee, Virginia M.-Y. Lee Departments of Pathology and Laboratory Medicine and Neurology University of Pennsylvania, Philadelphia, Pennsylvania 19104Search for more papers by this authorRobert W. Doms, Corresponding Author Robert W. Doms Departments of Pathology and Laboratory Medicine and Neurology University of Pennsylvania, Philadelphia, Pennsylvania 19104Department of Pathology and Laboratory Medicine, University of Pennsylvania, SCL-1, Rm. 512, 422 Curie Blvd., Philadelphia, PA 19104Search for more papers by this author First published: 28 October 1996 https://doi.org/10.1002/(SICI)1096-9861(19961028)374:4 3.0.CO;2-YCitations: 5AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract The human teratocarcinoma cell line NTera 2 (NT2) can be induced to differentiate into post-mitotic neurons possessing well-defined axonal and dendritic morphology. Highly enriched neurons (NT2-N cells) can be prepared in large numbers, thus combining many of the advantages of both primary and continuous cell culture systems. Unfortunately, it has proven difficult to express foreign genes in NT2-N cells. We examined whether vaccinia virus (VV) can express heterologous proteins in NT2-N cells and characterized the response of NT2-N cells to VV infection. NT2-N cells were infected with VV vectors expressing the envelope glycoprotein (gp160) from the human immunodeficiency type 1 virus (HIV-1). These vectors were chosen because VV-directed synthesis and post-translational processing of gp160 have been well characterized in many cell types. Approximately 85% of the neurons expressed gp160 which underwent native post-translational cleavage. The rate of gp160 synthesis was maximal at 5–48 hours postinfection, but was detectable for as long as 4 days. Surprisingly, NT2-N cells showed no VV-induced alterations in morphology, downregulation of host protein synthesis, or cytotoxicity, as measured by lactate dehydrogenase release. These results indicate that VV can serve as an efficient vector for introducing foreign genes in NT2-N cells without the cytotoxic effects often associated with VV infection. These properties, in conjunction with the advantages provided by NT2-N cells, provide new options for analyzing the cellular and molecular functions of human neurons. © 1996 Wiley-Liss, Inc. Citing Literature Volume374, Issue428 October 1996Pages 481-492 RelatedInformation