Synthesis of a fully active snake venom cardiotoxin by fragment condensation on solid polymer
1978; Elsevier BV; Volume: 536; Issue: 2 Linguagem: Inglês
10.1016/0005-2795(78)90496-8
ISSN1878-1454
AutoresChi‐Huey Wong, Shui‐Tien Chen, C.L. Ho, Kung‐Tsung Wang,
Tópico(s)Chemical Synthesis and Analysis
ResumoA polypeptide cardiotoxin containing 60 amino acids with 4 disulfide bonds has been synthesized by the "fragment solid-phase" method. The identity of the synthetic product with native cardiotoxin was established by chromatography on Sephadex G-50, carboxymethyl-cellulose column chromatography, thin layer chromatography, disc gel electrophoresis, amino acid analysis, end group analysis, peptide mapping, circular dichroism spectra, and four biological tests.
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