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Optimized Enzymatic Synthesis of Hesperidin Fatty Acid Esters in a Two-Phase System Containing Ionic Liquid

2011; Multidisciplinary Digital Publishing Institute; Volume: 16; Issue: 8 Linguagem: Inglês

10.3390/molecules16087171

1433-1373

Maria Elisa Melo Branco de Araújo, Fabiano Jares Contesini, Yollanda Edwirges Moreira Franco, Alexandra Christine Helena Frankland Sawaya, Thiago Grando Alberto, Natália Dalfré, Patrícia de Oliveira Carvalho,

Plant biochemistry and biosynthesis

Response surface methodology (RSM) based on a five-level, three-variable central composite design (CCD) was employed for modeling and optimizing the conversion yield of the enzymatic acylation of hesperidin with decanoic acid using immobilized Candida antarctica lipase B (CALB) in a two-phase system containing [bmim]BF4. The three variables studied (molar ratio of hesperidin to decanoic acid, [bmim]BF4/acetone ratio and lipase concentration) significantly affected the conversion yield of acylated hesperidin derivative. Verification experiments confirmed the validity of the predicted model. The lipase showed higher conversion degree in a two-phase system using [bmim]BF4 and acetone compared to that in pure acetone. Under the optimal reaction conditions carried out in a single-step biocatalytic process when the water content was kept lower than 200 ppm, the maximum acylation yield was 53.6%.