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Response of isolated nuclei to phospholipid vescicles: Analysis of chromatin sensitivity to DNase I and micrococcal nuclease

1984; Academic Press; Volume: 8; Issue: 1 Linguagem: Inglês

10.1016/0309-1651(84)90182-6

1878-240X

Lucio Cocco, Ryan Gilmour, Stefano Papa, Silvano Capitani, F.A. Manzoli,

Cell death mechanisms and regulation

Phosphatidylserine (PS) and phosphatidylcholine (PC) multilamellar vesicles (MLV) affect chromatin structure as analysed by DNase I sensitivity. The kinetics of DNA solubilisation during the digestion of nuclei indicates that phosphatidylserine causes an increase in DNase accessibility while phosphatidylcholine slightly reduces this accessibility. The effect of phosphatidylserine has also been analysed by means of isokinetic sucrose gradients and agarose gel electrophoresis of nuclear DNA solubilised by micrococcal nuclease. This analysis indicates that phosphatidylserine induces a very rapid production of mononucleosome subunits as compared with untreated nuclei.