Fluorescence-lifetime-based tomography for turbid media
2005; Optica Publishing Group; Volume: 30; Issue: 24 Linguagem: Inglês
10.1364/ol.30.003347
ISSN1539-4794
AutoresAnand T. N. Kumar, Jesse Skoch, Brian J. Bacskai, David A. Boas, Andrew K. Dunn,
Tópico(s)Advanced Fluorescence Microscopy Techniques
ResumoWe derive a novel algorithm to recover the in vivo distributions of fluorophores based on an asymptotic lifetime analysis of time-domain fluorescence measurements with turbid tissue. We experimentally demonstrate the advantage offered by this method in localizing fluorophores with distinct lifetimes. This algorithm has wide applicability for diagnostic fluorescence imaging in the presence of several-centimeter-thick biological tissue, since fluorescence lifetime is a sensitive indicator of local tissue environment and interactions at the molecular level.
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