Overexpression of T‐type calcium channels in HEK‐293 cells increases intracellular calcium without affecting cellular proliferation
2000; Wiley; Volume: 478; Issue: 1-2 Linguagem: Inglês
10.1016/s0014-5793(00)01832-9
ISSN1873-3468
AutoresJean Chemin, Arnaud Monteil, Christelle Briquaire, Sylvain Richard, Edward Perez‐Reyes, Joël Nargeot, Philippe Lory,
Tópico(s)Ion Channels and Receptors
ResumoIncreased expression of low voltage‐activated, T‐type Ca 2+ channels has been correlated with a variety of cellular events including cell proliferation and cell cycle kinetics. The recent cloning of three genes encoding T‐type α 1 subunits, α 1G , α 1H and α 1I , now allows direct assessment of their involvement in mediating cellular proliferation. By overexpressing the human α 1G and α 1H subunits in human embryonic kidney (HEK‐293) cells, we describe here that, although T‐type channels mediate increases in intracellular Ca 2+ concentrations, there is no significant change in bromodeoxyuridine incorporation and flow cytometric analysis. These results demonstrate that expressions of T‐type Ca 2+ channels are not sufficient to modulate cellular proliferation of HEK‐293 cells.
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