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BIBTEX RIS

Increased DNA binding and sequence discrimination of PNA oligomers containing 2,6-diaminopurine

1997; Oxford University Press; Volume: 25; Issue: 22 Linguagem: Inglês

10.1093/nar/25.22.4639

ISSN

1362-4962

Autores

Gerald Haaima,

Tópico(s)

Polyomavirus and related diseases

Resumo

The synthesis of a diaminopurine PNA monomer, N -[ N6 -(benzyloxycarbonyl)-2,6-diaminopurine-9-yl] acetyl- N -(2-t-butyloxycarbonylaminoethyl)glycine, and the incorporation of this monomer into PNA oligomers are described. Substitution of adenine by diaminopurine in PNA oligomers increased the Tm of duplexes formed with complementary DNA, RNA or PNA by 2.5–6.5°C per diaminopurine. Furthermore, discrimination against mismatches facing the diaminopurine in the hybridizing oligomer is improved. Finally, a homopurine decamer PNA containing six diaminopurines is shown to form a (gel shift) stable strand displacement complex with a target in a 246 bp double-stranded DNA fragment.

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