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In situ isolation of allelochemicals released from soft corals (Coelenterata : Octocorallia): A totally submersible sampling apparatus

1982; Elsevier BV; Volume: 60; Issue: 2-3 Linguagem: Inglês

10.1016/0022-0981(82)90166-6

1879-1697

John C. Coll, Bruce F. Bowden, Dianne M. Tapiolas, Walter C. Dunlap,

Marine Biology and Ecology Research

A submersible apparatus has been developed which permits in situ sampling of allelochemical substances released from sessile marine organisms. Concentration of the allomones from sea water is achieved by adsorption on SEP-PAK C-18, reverse-phase cartridges. The procedure is performed under water and causes minimal disturbance to the organism while in its natural habitat. Design and application of this apparatus are detailed. The isolation and identification of toxins released by two species of alcyonarian corals (order Alcyonacea) are described. This represents the first direct in situ isolation of water-borne allelochemicals released from a marine organism.