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RNase-sensitive glucocorticoid-receptor complexes from HeLa cell nuclei

1983; Elsevier BV; Volume: 113; Issue: 3 Linguagem: Inglês

10.1016/0006-291x(83)91080-x

1090-2104

Gian Paolo Rossini, Bruno Barbiroli,

thermodynamics and calorimetric analyses

Dexamethasone-receptor complexes can be extracted from HeLa cell nuclei by mild sonication. These complexes can account for 800-1000 binding sites/cell, and are indistinguishable from salt-extractable ones as judged by sucrose gradient centrifugation in the presence of 0.3 M KCl, showing a sedimentation coefficient of 3.6 S. These complexes, however, broadly sediment in the 7 to 3.6 S region of low salt sucrose gradients. Enzymatic treatment of soluble extracts from nuclear sonicates shows that RNA is associated to dexamethasone-receptor complexes.