Effects of pertussis toxin on delayed-type hypersensitivity responses and on the activity of suppressor T cells on the responses
1983; Elsevier BV; Volume: 81; Issue: 2 Linguagem: Inglês
10.1016/0008-8749(83)90230-7
ISSN1090-2163
AutoresShinichi Tamura, Yoko Nakanishi, Asato Kojima, Minoru Otokawa, Nobuyuki Uchida, Hiroko Sato, Yuji Sato,
Tópico(s)Monoclonal and Polyclonal Antibodies Research
ResumoExperiments were performed on mice to investigate the effects of pertussis toxin (PT) on delayed-type hypersensitivity (DTH) to ovalbumin (OA) and on the activity of suppressor T cells on the DTH (DTH-Ts). Mice immunized with alum-precipitated ovalbumin showed a transient DTH, which was determined as footpad swelling which disappeared 2 weeks after immunization. Maximal footpad swelling was observed 24 hr after DTH elicitation. On the other hand, when mice received PT (2 micrograms/mouse) at the time of immunization, the transient DTH became an enhanced and persistent DTH, which persisted for at least 4 weeks. In addition, the time of maximum footpad swelling was delayed from 24 to 48 hr after DTH elicitation. The immune spleen T cells from PT-treated mice showed a persistently high ability to transfer DTH into syngenic naive mice. DTH-Ts was induced in spleens of mice injected iv with OA-coupled syngeneic spleen cells. However, when these mice received PT at the time of suppressor induction, their spleen cells revealed considerably reduced suppressor activity. The activity of DTH-Ts was also reduced when DTH-Ts were either treated in vitro with PT or transferred into PT-injected recipient mice. From these results, interference with the suppressor function of DTH-Ts from PT was considered to be, at least in part, as an enhancing mechanism of DTH.
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