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Study of different coupling agents in the conjugation of a V3‐based synthetic MAP to carrier proteins

2001; Wiley; Volume: 7; Issue: 9 Linguagem: Inglês

10.1002/psc.336

1099-1387

Luis J. Cruz, Enrique V. Iglesias, Julio C. Aguilar, Diógenes Quintana, Hilda Garay, Carlos A. Duarte, Osvaldo Reyes,

HIV/AIDS drug development and treatment

The conjugation of synthetic peptides to carrier proteins is a widely used method for immunological studies. Different coupling agents have been described to form the conjugate with carrier proteins. In this paper, we demonstrate that the antibody response toward V3-based synthetic MAPs derived from HIV-1, JY1 isolate, conjugated to two different carrier proteins using either m-maleimidobenzoyl-N-hydroxysuccinimide ester (MBS) or beta-maleimidopropionic acid N-hydroxysuccinimide ester (MPS), or succinic anhydride (SA) show different behaviors. An excellent anti-JY1 response without a strong response to the coupling agent is observed in the case of succinic anhydride spacer. In contrast, MBS produces total abrogation of the antibody response with a high response toward the coupling agent.