Validity of Martin's equation in reversed‐phase paper‐chromatography
1965; Royal Netherlands Chemical Society; Volume: 84; Issue: 5 Linguagem: Inglês
10.1002/recl.19650840523
ISSN1878-7096
Autores Tópico(s)Crystallization and Solubility Studies
ResumoAbstract The properties of the paper‐chromatographic system paraffin oil/acetic acid‐water (84 + 16), applied to the analysis of sterols, have been investigated. The variation of the cross sectional areas occupied by the stationary and mobile phases (A L /A S ratio) was studied. By inserting experimental values for A L /A S , R F and the partition coefficients of two sterols in the equation of Martin : α = A L /A S (1/R F ‐ 1), strong evidence was obtained as to the validity of this equation in the reversed‐phase system studied.
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