Genetic Polymorphism in Caseins of Cow's Milk. V. End-Group Analysis of β-Caseins A, B, and C
1965; Elsevier BV; Volume: 48; Issue: 7 Linguagem: Inglês
10.3168/jds.s0022-0302(65)88356-4
ISSN1529-9066
AutoresEdwin B. Kalan, M.P. Thompson, Rae Greenberg, L. Pepper,
Tópico(s)Animal Genetics and Reproduction
ResumoThe three known genetic variants of fl-casein (A, B, and C) have been purified by colunm chromatography in 3.3 M urea, and their purity verified by polyacDqamide-gel electrophoresis.Qualitative determination of the aminoternlinal amino acid by two independent chemical methods revealed arginine for each of the three variants.During reaction with carboxypeptidase A, valine was reteased most rapidly and was closely followed by isoleucine.The data indicate either an ileu-ileu-val or an ileu-val-ileu C-terminal sequence in each variant.Molecular weights of about 25,000 for fl-caseins A, B, and C were calculated from the amount of valine released.
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