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Relative orientation of close-packed β-pleated sheets in proteins

1981; National Academy of Sciences; Volume: 78; Issue: 7 Linguagem: Inglês

10.1073/pnas.78.7.4146

1091-6490

Cyrus Chothia, Joël Janin,

Protein purification and stability

When beta-pleated sheets pack face to face in proteins, the angle between the strand directions of the two beta-sheets is observed to be near -30 degrees . We propose a simple model for beta-sheet-to-beta-sheet packing in concanavalin A, plastocyanin, gamma-crystallin, superoxide dismutase, prealbumin, and the immunoglobin fragment V(REI). This model shows how the observed relative orientation of two packed beta-sheets is a consequence of (i) the rows of side chains at the interface being approximately aligned and (ii) the beta-sheet having a right-handed twist. The special amino acid composition of residues at the beta-sheet-to-beta-sheet interfaces makes the contact surfaces essentially smooth and hydrophobic.