Quantitative Determination of Lactase
1961; Elsevier BV; Volume: 44; Issue: 3 Linguagem: Inglês
10.3168/jds.s0022-0302(61)89755-5
ISSN1529-9066
AutoresLenore Jasewicz, Aaron E. Wasserman,
Tópico(s)Enzyme function and inhibition
ResumoA simple and quick assay is described for the determination of the lactase activity of crude enzyme preparations.Results are available in 2 hr.from as little as 2 rag. of material.Fifteen minutes' incubation at 37 ° C. is used for the enzymatic reaction on a pure lactose substrate.The lactase activity is related to the amount of glucose released by the hydrolysis, as determined by an enzymatic micro-method.The effects of substrate concentration, pit, temperature, and related factors are investigated.The assay procedure was advantageously employed in a preliminary quantitative screening of lactaseproducing organisms which could utilize whey in their growth.
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