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Cytotoxicity, acute and subchronic toxicity of ionic liquid, didecyldimethylammonium saccharinate, in rats

2010; Elsevier BV; Volume: 57; Issue: 2-3 Linguagem: Inglês

10.1016/j.yrtph.2010.03.006

1096-0295

Jadwiga Jodynis‐Liebert, Michał Nowicki, Marek Murias, Teresa Adamska, Małgorzata Ewertowska, Małgorzata Kujawska, H. Piotrowska, Aneta Konwerska, Danuta Ostalska‐Nowicka, Juliusz Pernak,

Analytical chemistry methods development

The aim of this study was to investigate cytotoxicity, acute and subchronic oral toxicity of an ionic liquid didecyldimethylammonium saccharinate [DDA][Sac] in rat. IC50 values tested on six human cell lines varied from 1.44 μM to 5.47 μM. The compound tested was classified to the 4th toxicity class with a fixed LD50 cut-off value 500 mg/kg. Organ pathology induced by [DDA][Sac] in an acute experiment included exfoliation of the surface layer of the colon and alveolar septa in lung parenchyma. In a subchronic experiment rats were administered 10, 30 and 100 mg/kg/day [DDA][Sac] for 28 days. Reduced body weight gain and slightly reduced food consumption was observed particularly in high-dose rats. Slight hematology changes were found only in mid-dose females. Statistically significant changes in clinical chemistry parameters included: increases in the ALT, SDH, ALP and GGT activities, and in glucose, blood urea nitrogen and creatinine concentrations. However, these changes did not occur in both sexes and were not dose-related with the exception of ALP in females. No treatment-related microscopic changes were observed in a subchronic experiment. Under the condition of this study the lowest-observed-adverse-effect level of [DDA][Sac] was considered to be 10 mg/kg/day.