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Mass action kinetics of virus-cell aggregation and fusion

1988; Elsevier BV; Volume: 54; Issue: 3 Linguagem: Inglês

10.1016/s0006-3495(88)82978-3

1542-0086

Joe Bentz, Shlomo Nir, David G. Covell,

Protein purification and stability

A simple approximate solution for the mass action kinetics of small particles (viruses or vesicles) binding to large particles (cells) and their subsequent fusion has been derived. The solution is evaluated in terms of the measurable fluorescence changes expected when the virus or vesicles are labeled with fluorescent probes, which are diluted into the cellular membrane by fusion. Comparison with numerical integrations shows that the approximate solution is extremely accurate. Analytic simplifications for a variety of special cases of this general problem are also shown.