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Genetic diversity and phylogeographic structure of sixteen Mediterranean chicken breeds assessed with microsatellites and mitochondrial DNA

2015; Elsevier BV; Volume: 175; Linguagem: Inglês

10.1016/j.livsci.2015.03.003

1878-0490

Simone Ceccobelli, Lorenzo Pisello, Hovirag Lancioni, Luis Vicente Monteagudo Ibáñez, María Teresa Tejedor, Cesare Castellini, Vincenzo Landi, Amparo Martínez, Juan Vicente Delgado Bermejo, J. L. Vega‐Pla, José Manuel León Jurado, Natalia C. García, George S. Attard, A. Grimal, Srđan Stojanović, K. Kume, F. Panella, Steffen Weigend, Emiliano Lasagna,

Silkworms and Sericulture Research

The genetic diversities and relationships among 16 local breeds of chicken originating from five countries (Italy, Spain, Serbia, Albania and Republic of Malta) within the Mediterranean basin were assessed by sequencing part of the mitochondrial DNA (mtDNA) D-loop region and by genotyping individuals at 27 autosomal microsatellite loci. The aim was to study the microevolution of chicken on the northern shores of the Mediterranean and to determine their present genetic status. A 506 bp fragment of the mtDNA control region was sequenced in 160 individual DNA samples. The mtDNA sequence polymorphisms nomenclature that is normally used in these studies suggests that the Mediterranean chicken breeds under investigation are related to haplogroup E. A total of 465 blood samples were collected and utilised for microsatellite analysis. Six breeds (Ancona, Livornese Bianca—Italy; Pita Pinta Asturiana, Gallina de Sobrarbe—Spain; Albanian population—Albania; and the Maltese Black—Malta) showed significant high levels of inbreeding. About 22% of the total genetic variation observed was due to variability between populations. STRUCTURE analysis confirmed the breed variability result (FST=0.22) also observed in the Neighbor-Net dendrogram. These results strongly allude that the 16 Mediterranean chicken breeds studied originated from three distinct maternal lineages and retain moderate levels of autosomal genetic diversity.