Contribution of phosphatidylinositol 3-kinase to radiation resistance in human melanoma cells
1999; Wiley; Volume: 24; Issue: 1 Linguagem: Inglês
10.1002/(sici)1098-2744(199901)24
ISSN1098-2744
AutoresKrasil'nikov Ma, Victor Adler, Serge Y. Fuchs, Zheng Dong, Adriana Haimovitz–Friedman, Meenhard Herlyn, Ze’ev A. Ronai,
Tópico(s)Genomics, phytochemicals, and oxidative stress
ResumoMolecular CarcinogenesisVolume 24, Issue 1 p. 64-69 Article Contribution of phosphatidylinositol 3-kinase to radiation resistance in human melanoma cells Mikhail Krasilnikov, Mikhail Krasilnikov Laboratory of Tumor Biochemistry, Institute of Carcinogenesis Cancer Research Center, Moscow, Russian FederationSearch for more papers by this authorVictor Adler, Victor Adler The Ruttenberg Cancer Center, Mount Sinai School of Medicine, New York, New YorkSearch for more papers by this authorSerge Y. Fuchs, Serge Y. Fuchs The Ruttenberg Cancer Center, Mount Sinai School of Medicine, New York, New YorkSearch for more papers by this authorZheng Dong, Zheng Dong The Ruttenberg Cancer Center, Mount Sinai School of Medicine, New York, New YorkSearch for more papers by this authorAdriana Haimovitz-Friedman, Adriana Haimovitz-Friedman Department of Radiation Biology, Memorial Sloan Kettering Cancer Center, New York, New YorkSearch for more papers by this authorMeenhard Herlyn, Meenhard Herlyn Program of Molecular and Cellular Biology, The Wistar Institute, Philadelphia, PennsylvaniaSearch for more papers by this authorZe'ev Ronai, Corresponding Author Ze'ev Ronai The Ruttenberg Cancer Center, Mount Sinai School of Medicine, New York, New YorkThe Ruttenberg Cancer Center, Mount Sinai School of Medicine, One Gustave L. Levy Place, Box 1130, New York, NY 10029-6574Search for more papers by this author Mikhail Krasilnikov, Mikhail Krasilnikov Laboratory of Tumor Biochemistry, Institute of Carcinogenesis Cancer Research Center, Moscow, Russian FederationSearch for more papers by this authorVictor Adler, Victor Adler The Ruttenberg Cancer Center, Mount Sinai School of Medicine, New York, New YorkSearch for more papers by this authorSerge Y. Fuchs, Serge Y. Fuchs The Ruttenberg Cancer Center, Mount Sinai School of Medicine, New York, New YorkSearch for more papers by this authorZheng Dong, Zheng Dong The Ruttenberg Cancer Center, Mount Sinai School of Medicine, New York, New YorkSearch for more papers by this authorAdriana Haimovitz-Friedman, Adriana Haimovitz-Friedman Department of Radiation Biology, Memorial Sloan Kettering Cancer Center, New York, New YorkSearch for more papers by this authorMeenhard Herlyn, Meenhard Herlyn Program of Molecular and Cellular Biology, The Wistar Institute, Philadelphia, PennsylvaniaSearch for more papers by this authorZe'ev Ronai, Corresponding Author Ze'ev Ronai The Ruttenberg Cancer Center, Mount Sinai School of Medicine, New York, New YorkThe Ruttenberg Cancer Center, Mount Sinai School of Medicine, One Gustave L. Levy Place, Box 1130, New York, NY 10029-6574Search for more papers by this author First published: 05 February 1999 https://doi.org/10.1002/(SICI)1098-2744(199901)24:1 3.0.CO;2-2Citations: 50AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract The activity of phosphatidylinositol 3-kinase (PI3K), a key component of multiple signal transduction pathways, was investigated in early- and late-stage melanoma cells that have varying degrees of radiation resistance. Analysis of PI3K biproducts (PI-3,4-P2 and PI-3,4,5-triphosphate) revealed a direct correlation between radiation resistance and levels of PI3K activity. Treating melanoma cells with wortmanin or LY294002, two different PI3K inhibitors, decreased PI3K activity and caused a dose-dependent decrease in resistance to ultraviolet radiation. Lower resistance to radiation elicited by LY294002 coincided with increased apoptosis. To further establish the role of PI3K in radiation resistance, we transfected early-stage melanoma cells with the cDNA of p85, the regulatory subunit of PI3K. Clones that constitutively overexpressed p85 exhibited a higher degree of PI-3,4-P2 synthesis and a corresponding increase in their resistance to ultraviolet radiation. The results of this study point to the role of PI3K and its biproducts in radiation resistance of human melanoma cells. Mol. Carcinog. 24:64–69, 1999. © 1999 Wiley-Liss, Inc. Citing Literature Volume24, Issue1January 1999Pages 64-69 RelatedInformation
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