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A Physical Map of the 1-Gigabase Bread Wheat Chromosome 3B

2008; American Association for the Advancement of Science; Volume: 322; Issue: 5898 Linguagem: Inglês

10.1126/science.1161847

1095-9203

Etienne Paux, Pierre Sourdille, Jérôme Salse, Cyrille Saintenac, Frédéric Choulet, Philippe Leroy, Abraham Korol, Monika Michalak, Shahryar F. Kianian, Wolfgang Spielmeyer, Evans Lagudah, Daryl J. Somers, Andrzej Kilian, Michaël Alaux, Sonia S. Vautrin, Hélène Bergès, Kellye Eversole, R. Appels, Jan Šafář, Hana Šimková, Jaroslav Doležel, Michel Bernard, Catherine Feuillet,

Wheat and Barley Genetics and Pathology

As the staple food for 35% of the world's population, wheat is one of the most important crop species. To date, sequence-based tools to accelerate wheat improvement are lacking. As part of the international effort to sequence the 17-billion-base-pair hexaploid bread wheat genome (2n = 6x = 42 chromosomes), we constructed a bacterial artificial chromosome (BAC)-based integrated physical map of the largest chromosome, 3B, that alone is 995 megabases. A chromosome-specific BAC library was used to assemble 82% of the chromosome into 1036 contigs that were anchored with 1443 molecular markers, providing a major resource for genetic and genomic studies. This physical map establishes a template for the remaining wheat chromosomes and demonstrates the feasibility of constructing physical maps in large, complex, polyploid genomes with a chromosome-based approach.