Authenticating Cell Lines in Ophthalmic Research Laboratories
2008; Cadmus Press; Volume: 49; Issue: 11 Linguagem: Inglês
10.1167/iovs.08-2324
ISSN1552-5783
AutoresRobert Folberg, Shrihari S. Kadkol, Shahar Frenkel, Klara Valyi‐Nagy, Martine J. Jager, Jacob Pe’er, Andrew J. Maniotis,
Tópico(s)Barrier Structure and Function Studies
ResumoAuthentication of cell lines in biomedical research has been elevated to a very high priority. From a review of the literature, Lacroix1 reviewed the issue of cross-contamination of cell lines including the well known contamination of cell lines with HeLa cells,2 and the mis-identification of the ECV304 cell line as “immortalized endothelial cells” when these cells in fact originated from T24 bladder carcinoma cells.3 Lacroix 1 estimated that between 18 and 36% of cell lines have been misclassified. One survey at a large research institution suggested that fewer than 50% of researchers authenticate their cell lines.4 Nardone5 proposed recently that identification of cell lines be required of investigators before grants are awarded, and the National Institutes of Health subsequently called for researchers to authenticate cell lines as a prerequisite for grant funding.6
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