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Nanoflow LC/Ion Mobility/CID/TOF for Proteomics: Analysis of a Human Urinary Proteome

2003; American Chemical Society; Volume: 2; Issue: 6 Linguagem: Inglês

10.1021/pr034018v

1535-3907

Myeong Hee Moon, Sunnie Myung, Manolo Plasencia, Amy E. Hilderbrand, David E. Clemmer,

Metabolomics and Mass Spectrometry Studies

A prototype linear octopole ion trap/ion mobility/tandem mass spectrometer has been coupled with a nanoflow liquid chromatography separation approach and used to separate and characterize a complicated peptide mixture from digestion of soluble proteins extracted from human urine. In this approach, two dimensions of separation (nanoflow liquid chromatography and ion mobility) are followed by collision induced dissociation (CID) and mass spectrometry (MS) analysis. From a preliminary analysis of the most intense CID−MS features in a part of the dataset, it is possible to assign 27 peptide ions which correspond to 13 proteins. The data contain many additional CID−MS features for less intense ions. A limited discussion of these features and their potential utility in identifying complicated peptide mixtures required for proteomics study is presented. Keywords: ion mobility separation • tandem mass spectrometry • nanoLC/IMS/MSMS • protein identification