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High-Frequency Secondary Mutations after Suicide-Driven Allelic Exchange Mutagenesis in Extraintestinal Pathogenic Escherichia coli

2003; American Society for Microbiology; Volume: 185; Issue: 17 Linguagem: Inglês

10.1128/jb.185.17.5301-5305.2003

1098-5530

James R. Johnson, Hank Lockman, Krista Owens, Srdjan Jelačić, Phillip I. Tarr,

Antibiotic Resistance in Bacteria

ABSTRACT Frequent unintended secondary mutations occurred in extraintestinal pathogenic Escherichia coli strains CP9, CFT073, and RS218 during suicide plasmid-mediated, putatively specific deletions of hlyA , papG allele III, and iha . Pulsed-field gel electrophoresis and PCR analyses demonstrated genomic alterations and/or unintended loss of defined virulence genes ( papG , the F7-2 papA allele, iutA , sat , hlyD , and cnf ). Caution is warranted when attributing the observed phenotypic changes to the intended mutation.