Protection against anthrax toxin by vaccination with a DNA plasmid encoding anthrax protective antigen
1999; Elsevier BV; Volume: 17; Issue: 4 Linguagem: Inglês
10.1016/s0264-410x(98)00210-2
ISSN1873-2518
AutoresMili Gu, Stephen H. Leppla, Dennis M. Klinman,
Tópico(s)Bacterial Genetics and Biotechnology
ResumoA DNA vaccine encoding the immunogenic and biologically active portion of anthrax protective antigen (PA) was constructed. Spleen cells from BALB/c mice immunized intramuscularly with this vaccine were stimulated to secrete IFNγ and IL-4 when exposed to PA in vitro. Immunized mice also mounted a humoral immune response dominated by IgG1 anti-PA antibody production, the subclass previously shown to confer protection against anthrax toxin. A 1:100 dilution of serum from these animals protected cells in vitro against cytotoxic concentrations of PA. Moreover, 7/8 mice immunized three times with the PA DNA vaccine were protected against lethal challenge with a combination of anthrax protective antigen plus lethal factor.
Referência(s)