Superiority of Bone Marrow-Derived Dendritic Cells Over Monocyte-Derived Ones for the Expansion of Regulatory T Cells in the Macaque
2008; Wolters Kluwer; Volume: 85; Issue: 9 Linguagem: Inglês
10.1097/tp.0b013e31816f22d6
ISSN1534-6080
AutoresAurélie Moreau, Elise Chiffoleau, Gaëlle Bériou, Jack‐Yves Deschamps, Michèle Heslan, Joanna Ashton‐Chess, Fabienne Rolling, Régis Josien, Philippe Moullier, María Cristina Cuturi, Brigitte Alliot‐Licht,
Tópico(s)Immune Cell Function and Interaction
ResumoRegulatory T cells (Treg) have been identified as playing a pivotal role in the control of tolerance and in the suppression of pathologic immune responses in autoimmune diseases, transplantation, and graft-versus-host disease. Treg expanded ex vivo by dendritic cells could be potential reagents to promote antigen-specific tolerance in vivo. However, in vivo studies have been carried out mostly in rodents and will need validation in primates before clinical application. We characterized macaque dendritic cell derived either from bone marrow with and without prior CD34+ cell selection (BMDC), or from CD14+ peripheral blood mononuclear cells (Mo-DC). We demonstrate that with a semi-mature phenotype, BMDC are superior to Mo-DC in their capacity to expand freshly isolated allogeneic macaque CD4+CD25+CD127−Foxp3+Treg in vitro in the presence of interleukin-2. Moreover, the expanded Treg maintain their phenotype and suppressive activity. These data provide a step toward the use of macaque dendritic cell to expand Treg for future preclinical testing.
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