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Local Expression of Cytokine Messenger RNA in Rat Model of Escherichia Coli Epididymitis

1995; Lippincott Williams & Wilkins; Volume: 154; Issue: 6 Linguagem: Inglês

10.1016/s0022-5347(01)66724-7

1527-3792

Kazushi Tanaka, Masato Fujisawa, Soichi Arakawa, Sadao Kamidono,

Reproductive System and Pregnancy

No AccessJournal of UrologyInvestigative Urology1 Dec 1995Local Expression of Cytokine Messenger RNA in Rat Model of Escherichia Coli Epididymitis Kazushi* Tanaka, Masato Fujisawa, Soichi Arakawa, and Sadao Kamidono Kazushi* TanakaKazushi* Tanaka , Masato FujisawaMasato Fujisawa , Soichi ArakawaSoichi Arakawa , and Sadao KamidonoSadao Kamidono View All Author Informationhttps://doi.org/10.1016/S0022-5347(01)66724-7AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: In order to investigate whether cytokines play an important role in the regulation of host defense against bacterial epididymitis, we examined the local expression of cytokine mRNA in a rat model of Escherichia coli epididymitis. Materials and Methods: Escherichia coli was inoculated into the spermatic cord of the rats and provoked epididymitis. At 6, 12, 24, 48, and 72 hours postinfection (p.i.) epididymides were harvested. We examined interleukin-6 (IL-6) and other inflammatory cytokine mRNAs in epididymides by Northern blotting and reverse transcriptase polymerase chain reaction (RT-PCR). Histopathological findings and immunohistochemistry for IL-6 were also examined. Results: Infected epididymides expressed IL-6 mRNA maximally at 6 hours p.i. and then the signal decreased by Northern blotting. mRNA for IL-1 beta and TNF-alpha also increased within 6 hours by RT-PCR. Histopathological examination showed that no inflammatory cells were observed at 6 hours p.i. and that inflammatory changes were most prominent at 72 hours p.i. Epididymal epithelium expressed IL-6 locally in response to local bacterial infection by immunohistochemistry. Conclusions: mRNA for IL-1 beta, IL-6, and TNF-alpha is expressed locally in response to bacterial infection before inflammatory cell infiltration. This rapid expression in the epithelial cells may be important in host defense to local genital infection. References 1 : The cytokine network in the critically ill.. Anaesth. Intensive Care1992; 20: 288. Google Scholar 2 : Interferon beta sub 2 /B-cell stimulatory factor type 2 shares identity with monocyte-derived hepatocyte-stimulating factor and regulates the major acute phase protein response in liver cells.. Proc. Natl. Acad. Sci. U.S.A.1987; 84: 7251. Google Scholar 3 : Interleukin 6 is involved in interleukin 1-induced activities.. Eur. J. Immunol.1988; 18: 957. Google Scholar 4 : The essential role of B cell stimulatory factor 2 (BSF-2/IL-6) for the terminal differentiation of B cells.. J. Exp. Med.1988; 167: 332. Google Scholar 5 : Interleukins and IgA synthesis.. Human and murine interleukin 6 induce high rate IgA secretion in IgA-committed B cells. J. Exp. Med.1989; 169: 2133. Google Scholar 6 : Stimulation of antibacterial macrophage activities by B-cell stimulatory factor 2 (interleukin-6).. Infect. Immun.1990; 58: 269. Google Scholar 7 : Protective role of interleukin 6 in the lipopolysaccharide-galactosamine septic shock model.. Infect. Immun.1993; 61: 1496. Google Scholar 8 : Recombinant interleukin-6 protects mice against experimental bacterial infection.. Infect. Immun.1992; 60: 4402. Google Scholar 9 : Local cytokine production in a murine model of Escherichia coli pyelonephritis.. J. Clin. Invest.1992; 89: 1032. Google Scholar 10 : Local production of tumor necrosis factor alpha, interleukin 1, and interleukin 6 in meningococcal meningitis.. Relation to the inflammatory response. J. Exp. Med.1989; 170: 1859. Google Scholar 11 : The complex pattern of cytokines in serum from patients with meningococcal septic shock. Association between interleukin 6, interleukin 1, and fatal outcome.. J. Exp. Med.1989; 169: 333. Google Scholar 12 : Tumor necrosis factor and interleukin-1 in the serum of children with severe infectious purpura.. N. Engl. J. Med.1988; 319: 397. Google Scholar 13 : Interleukin-6 response to deliberate colonization of the human urinary tract with Gram-negative bacteria.. Infect. Immun.1991; 59: 421. Google Scholar 14 : Interleukin-6 induced at mucosal surfaces by Gram-negative bacterial infection.. Infect. Immun.1989; 57: 3383. Google Scholar 15 : Effect of gamma interferon on resolution of murine Chlamydial genital infection.. Infect. Immun.1992; 60: 4427. Google Scholar 16 : Experimental Escherichia coli epididymitis in rabbits.. Urology1988; 32: 236. Google Scholar 17 : Experimental epididymitis due to Chlamydia trachomatis in rats.. Infect. Immun.1992; 60: 2324. Google Scholar 18 : Bacterial epididymitis in the rat: a model for assessing the impact of acute inflammation on epididymal antibiotic penetration.. J. Urol.1990; 144: 780. Abstract, Google Scholar 19 : Acute experimental E.. coli epididymitis in the rat and its consequences on spermatogenesis. Urol. Res.1983; 11: 117. Google Scholar 20 : Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.. Anal. Biochem.1987; 162: 156. Google Scholar 21 : Structure of the rat interleukin 6 gene and its expression in macrophage-derived cells.. J. Biol. Chem.1989; 264: 16072. Google Scholar 22 : Effects of kinic acid on messenger RNA levels of IL-1 beta, IL-6, TNF alpha and LIF in the rat brain.. Biochem. Biophys. Res. Commun.1991; 176: 593. Google Scholar 23 : Interleukin-6 (IL-6) mRNAs expressed in Leydig and Sertoli cells are regulated by cytokines, gonadotropins and neuropeptides.. Endocrine1994; 2: 617. Google Scholar 24 : Local production of tumor necrosis factor and IFN-gamma in tuberculous pleuritis.. J. Immunol.1990; 145: 149. Google Scholar 25 : Interleukin-6 response of epithelial cell lines to bacterial stimulation in vitro.. Infect. Immun.1992; 60: 1259. Google Scholar 26 : Adhesion-dependent activation of mucosal interleukin-6 production.. Infect. Immun.1991; 59: 4357. Google Scholar 27 : Selective cytokine production by epithelial cells following exposure to Escherichia coli.. Infect. Immun.1993; 61: 602. Google Scholar 28 : Comparison of urine and serum concentrations of interleukin-6 in women with acute pyelonephritis or asymptomatic bacteriuria.. J. Infect. Dis.1992; 166: 653. Google Scholar Department of Urology, Kobe University School of Medicine, Kobe, Japan.© 1995 by American Urological Association, Inc.FiguresReferencesRelatedDetails Volume 154Issue 6December 1995Page: 2179-2184 Advertisement Copyright & Permissions© 1995 by American Urological Association, Inc.MetricsAuthor Information Kazushi* Tanaka More articles by this author Masato Fujisawa More articles by this author Soichi Arakawa More articles by this author Sadao Kamidono More articles by this author Expand All Advertisement PDF downloadLoading ...