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Enhancement in hydrogen production by co-cultures of Bacillus and Enterobacter

2014; Elsevier BV; Volume: 39; Issue: 27 Linguagem: Inglês

10.1016/j.ijhydene.2014.07.084

1879-3487

Sanjay K. S. Patel, Prasun Kumar, Sanjeet Mehariya, Hemant J. Purohit, Jung-Kul Lee, Vipin Chandra Kalia,

Microbial Fuel Cells and Bioremediation

Defined co-cultures of hydrogen (H2) producers belonging to Citrobacter, Enterobacter, Klebsiella and Bacillus were used for enhancing the efficiency of biological H2 production. Out of 11 co-cultures consisting of 2–4 strains, two co-cultures composed of Bacillus cereus EGU43, Enterobacter cloacae HPC123, and Klebsiella sp. HPC793 resulted in H2 yield up to 3.0 mol mol−1 of glucose. Up-scaling of the reactor by 16-fold resulted in a corresponding increase in H2 production with an actual evolution of 7.44 L of H2. It constituted 58.2% of the total biogas. Continuous culture evolution of H2 by co-cultures (B. cereus EGU43 and E. cloacae HPC123) immobilized on ligno-cellulosic materials resulted in 6.4-fold improvement in H2 yield compared to free floating bacteria. This synergistic influence of B. cereus and E. cloacae can offer a better strategy for H2 production than undefined or mixed cultures.