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Extracellular matrix expression and periodontal wound‐healing dynamics following guided tissue regeneration therapy in canine furcation defects

2007; Wiley; Volume: 34; Issue: 8 Linguagem: Inglês

10.1111/j.1600-051x.2007.01097.x

1600-051X

M. Christgau, Raúl G. Caffesse, Gottfried Schmalz, Rena N. D’Souza,

dental development and anomalies

Abstract Aim: Temporal and spatial expression pattern of extracellular matrix (ECM) components in furcation defects following guided tissue regeneration (GTR) compared with open‐flap debridement (OFD). Material and Methods: In 21 dogs, mandibular second and fourth pre‐molars were treated with one non‐resorbable and three different resorbable membranes. Third pre‐molars were treated by OFD. After 2, 4, 8 weeks and 3, 6, and 12 months, tissues were analysed by immunohistochemistry for collagen I (Col‐I) and III (Col‐III), fibronectin (FN), bone sialoprotein (BSP), and osteopontin (OPN). Results: At 2 weeks, the defect was mainly occupied by FN+ granulation tissue (GT), which was sequentially replaced by new connective tissue expressing FN, Col‐I, and increasingly Col‐III. Following superficial resorptions by OPN+ osteoclasts and odontoclasts, cementum and bone formation ensued with strong expression of BSP and OPN along bone and tooth surfaces. Deposition of Col‐I, FN, BSP and OPN+ cementoid and osteoid became evident after 4 weeks. Extrinsic fibres of cementum and bone stained intensely for Col‐III. The newly formed periodontal ligament expressed FN, Col‐I, and Col‐III, but no BSP or OPN. Conclusions: The spatial ECM expression was similar for OFD and the different GTR methods, although the timing and quantity of ECM expression were influenced by wound stabilization and inflammatory reactions.