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Detection of Cyclospora cayetanensis in travellers returning from the tropics and subtropics using microscopy and real-time PCR

2003; Elsevier BV; Volume: 293; Issue: 2-3 Linguagem: Inglês

10.1078/1438-4221-00252

1618-0607

Jaco J. Verweij, Daphne Laeijendecker, Eric A. T. Brienen, Lisette van Lieshout, Anton M. Polderman,

Viral gastroenteritis research and epidemiology

We examined 100 stool specimens of returning travellers with diarrhoea for the presence of Cyclospora cayetanensis using fluorescence microscopy and real-time PCR. C. cayetanensis was found in four cases with microscopy and PCR. One additional sample was positive only by PCR, and could be confirmed by microscopic examination of several additional slides. C. cayetanensis was the most frequent parasitic cause of diarrhoea after Giardia duodenalis.