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Exploration of the pore structure of a peptide-gated Na+ channel

2001; Springer Nature; Volume: 20; Issue: 20 Linguagem: Inglês

10.1093/emboj/20.20.5595

1460-2075

Mallorie Poët, Michel Tauc, Éric Lingueglia, Peggy Cance, Philippe Poujeol, Michel Lazdunski, Laurent Counillon,

Ion Transport and Channel Regulation

Article15 October 2001free access Exploration of the pore structure of a peptide-gated Na+ channel Mallorie Poët Mallorie Poët Laboratoire de Physiologie Cellulaire et Moléculaire, CNRS UMR6548, Université de Nice–Sophia Antipolis, Faculté des Sciences, Parc Valrose, 06108 Nice, France Search for more papers by this author Michel Tauc Michel Tauc Laboratoire de Physiologie Cellulaire et Moléculaire, CNRS UMR6548, Université de Nice–Sophia Antipolis, Faculté des Sciences, Parc Valrose, 06108 Nice, France Search for more papers by this author Eric Lingueglia Eric Lingueglia Institut de Pharmacologie Moléculaire et Cellulaire, CNRS UMR6097, Université de Nice–Sophia Antipolis, 660 route des Lucioles, 06560 Valbonne, France Search for more papers by this author Peggy Cance Peggy Cance Laboratoire de Physiologie Cellulaire et Moléculaire, CNRS UMR6548, Université de Nice–Sophia Antipolis, Faculté des Sciences, Parc Valrose, 06108 Nice, France Search for more papers by this author Philippe Poujeol Philippe Poujeol Laboratoire de Physiologie Cellulaire et Moléculaire, CNRS UMR6548, Université de Nice–Sophia Antipolis, Faculté des Sciences, Parc Valrose, 06108 Nice, France Search for more papers by this author Michel Lazdunski Michel Lazdunski Institut de Pharmacologie Moléculaire et Cellulaire, CNRS UMR6097, Université de Nice–Sophia Antipolis, 660 route des Lucioles, 06560 Valbonne, France Search for more papers by this author Laurent Counillon Corresponding Author Laurent Counillon Laboratoire de Physiologie Cellulaire et Moléculaire, CNRS UMR6548, Université de Nice–Sophia Antipolis, Faculté des Sciences, Parc Valrose, 06108 Nice, France Search for more papers by this author Mallorie Poët Mallorie Poët Laboratoire de Physiologie Cellulaire et Moléculaire, CNRS UMR6548, Université de Nice–Sophia Antipolis, Faculté des Sciences, Parc Valrose, 06108 Nice, France Search for more papers by this author Michel Tauc Michel Tauc Laboratoire de Physiologie Cellulaire et Moléculaire, CNRS UMR6548, Université de Nice–Sophia Antipolis, Faculté des Sciences, Parc Valrose, 06108 Nice, France Search for more papers by this author Eric Lingueglia Eric Lingueglia Institut de Pharmacologie Moléculaire et Cellulaire, CNRS UMR6097, Université de Nice–Sophia Antipolis, 660 route des Lucioles, 06560 Valbonne, France Search for more papers by this author Peggy Cance Peggy Cance Laboratoire de Physiologie Cellulaire et Moléculaire, CNRS UMR6548, Université de Nice–Sophia Antipolis, Faculté des Sciences, Parc Valrose, 06108 Nice, France Search for more papers by this author Philippe Poujeol Philippe Poujeol Laboratoire de Physiologie Cellulaire et Moléculaire, CNRS UMR6548, Université de Nice–Sophia Antipolis, Faculté des Sciences, Parc Valrose, 06108 Nice, France Search for more papers by this author Michel Lazdunski Michel Lazdunski Institut de Pharmacologie Moléculaire et Cellulaire, CNRS UMR6097, Université de Nice–Sophia Antipolis, 660 route des Lucioles, 06560 Valbonne, France Search for more papers by this author Laurent Counillon Corresponding Author Laurent Counillon Laboratoire de Physiologie Cellulaire et Moléculaire, CNRS UMR6548, Université de Nice–Sophia Antipolis, Faculté des Sciences, Parc Valrose, 06108 Nice, France Search for more papers by this author Author Information Mallorie Poët1, Michel Tauc1, Eric Lingueglia2, Peggy Cance1, Philippe Poujeol1, Michel Lazdunski2 and Laurent Counillon 1 1Laboratoire de Physiologie Cellulaire et Moléculaire, CNRS UMR6548, Université de Nice–Sophia Antipolis, Faculté des Sciences, Parc Valrose, 06108 Nice, France 2Institut de Pharmacologie Moléculaire et Cellulaire, CNRS UMR6097, Université de Nice–Sophia Antipolis, 660 route des Lucioles, 06560 Valbonne, France *Corresponding author. E-mail: [email protected] The EMBO Journal (2001)20:5595-5602https://doi.org/10.1093/emboj/20.20.5595 PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info The FMRF-amide-activated sodium channel (FaNaC), a member of the ENaC/Degenerin family, is a homotetramer, each subunit containing two transmembrane segments. We changed independently every residue of the first transmembrane segment (TM1) into a cysteine and tested each position's accessibility to the cysteine covalent reagents MTSET and MTSES. Eleven mutants were accessible to the cationic MTSET, showing that TM1 faces the ion translocation pathway. This was confirmed by the accessibility of cysteines present in the acid-sensing ion channels and other mutations introduced in FaNaC TM1. Modification of accessibilities for positions 69, 71 and 72 in the open state shows that the gating mechanism consists of the opening of a constriction close to the intracellular side. The anionic MTSES did not penetrate into the channel, indicating the presence of a charge selectivity filter in the outer vestibule. Furthermore, amiloride inhibition resulted in the channel occlusion in the middle of the pore. Summarizing, the ionic pore of FaNaC includes a large aqueous cavity, with a charge selectivity filter in the outer vestibule and the gate close to the interior. Introduction FaNaC is a member of the epithelial Na+ channel (ENaC)/Degenerin gene family of amiloride-sensitive cationic channels. These channels are involved in a wide variety of physiological functions, such as mechanosensation in Caenorhabditis elegans (degenerins; Tavernarakis and Driscoll, 1997), and maintenance of mammalian salt balance and salty taste perception (ENaC; Garty and Palmer, 1997) or acid sensing (acid-sensing ion channels, ASICs; Waldmann and Lazdunski, 1998). Mutations in genes encoding members of this family have been shown to be responsible for genetic disorders such as neurodegeneration in C.elegans (Chalfie and Wolinski, 1990; Driscoll and Chalfie, 1991) or chronic hypertension (Liddle's syndrome; Shimkets et al., 1994; Hansson et al., 1995) and pseudohypoaldosteronism type 1 in man (Chang et al., 1996). FaNaC has been discovered in Helix aspersa neurons (Green et al., 1994; Cottrell, 1997) and it has the unique property to form a Na+-selective ion channel directly activated by the Phe-Met-Arg-Phe-NH2 (FMRF-amide) peptide. The functional unit of FaNaC is a homotetramer (Coscoy et al., 1998), each subunit consisting of two transmembrane segments connected by a large cysteine-rich loop shown to be extracellular in other ENaC/Degenerin channels (Canessa et al., 1994; Renard et al., 1994; Lai et al., 1996). Although no mammalian FaNaC has yet been isolated, this channel is evolutionarily related to the mammalian ASICs, which are external pH sensors present in brain and in sensory neurons where they are thought to participate in nociception associated with tissue acidosis, for instance in ischemic, damaged or inflamed tissue (Waldmann and Lazdunski, 1998). A functional modulation of certain ASIC channels by FMRF-amide and related peptides was recently described (Askwith et al., 2000). Due to its unique properties, we decided to use FaNaC as a model system to learn more about the structure–function relationships of the ligand-gated channels belonging to the ENaC/Degenerin gene family. We used the substituted-cysteine accessibility method (Akabas et al., 1992) to investigate the participation of the first transmembrane segment in the ion pore of FaNaC. Each residue of the first transmembrane segment has been substituted individually into a cysteine, and each functional mutant has been exposed to methanethiosulfonates (MTS), i.e. polar cysteine covalent reagents. These hydrophilic molecules can only modify cysteines situated in the aqueous lumen of the channel, because these reagents are charged, and because their reactivity is negligible for thiol groups exposed to a non-polar environment (for review see Karlin and Akabas, 1998). Therefore, this method allows the identification of the residues of transmembrane segments lining the ion translocation pathway. In addition, the pattern of modification provides clues to the secondary structure of these segments. This procedure can also be applied to the TM2. However, its high level of conservation suggests that the systematic substitution of residues into cysteines would lead to the generation of many inactive mutants. The obtainment of 11 inactive cysteine mutants of TM2 in an independent work (M.Poët, P.Cance and L.Counillon, unpublished results) confirms this assumption. In contrast, the high tolerance of TM1 for amino acid substitutions enabled us to use this segment as a reporter for the characterization of the FaNaC ion pore. Results Expression and characterization of wild-type FaNaC The cDNA encoding FaNaC was introduced in the pIRES-CD8 polycistronic vector, which allows the coexpression of the cell surface marker CD8 and of the cDNA of interest. Two days after transfection, HEK293 cells containing the pIRES-CD8 plasmid encoding the wild-type FaNaC could be detected visually by the binding of anti-CD8 monoclonal antibody-coated beads (Dynal), and were tested using the whole-cell clamp technique. They expressed a typical FMRF-amide-activated amiloride-sensitive sodium conductance with a Kd for FMRF-amide of 1 μM, and a Ki for amiloride of 8 μM, in good agreement with previous data (Lingueglia et al., 1995; Coscoy et al., 1998). This current, which exhibited a typical current–voltage relationship, was not present in non-transfected cells, and did not exhibit significant desensitization upon repetitive applications of FMRF-amide (Figures 1B and 2). Figure 1.(A) Sequence alignments for TM1 and TM2 regions within different members of the ENaC/Degenerin gene family. The alignment was obtained with the GCG Pileup program. Identical and similar residues are printed white on black and white on gray, respectively. Acid-sensing ion channels (ASICs), brain–liver–intestine amiloride-sensitive Na+ channel (BLINaC) and epithelial Na+ channel (ENaC) correspond to the rat sequences; MEC4 and UNC105 are C.elegans degenerins and dGNaC1 is a Drosophila gonad-specific amiloride-sensitive Na+ channel. Note the low conservation between the sequences of TM1 when compared with TM2. (B) Current–voltage relationship for wild-type FaNaC expressed in HEK293 cells, in the presence of 30 μM FMRF-amide. Cells were maintained in the whole-cell configuration and voltage steps were applied from −100 to +100 mV in 20 mV increments. (Circles) Symmetrical conditions (140 mM NaCl, 0 mM KCl pH 7.4) between intracellular and extracellular medium. (Squares) Extracellular medium: 95 mM KCl, 35 mM NaCl; intracellular medium: 140 mM NaCl, 0 mM KCl. (Diamonds) Residual currents in the absence of FMRF-amide. Similar residual currents were observed in the presence of 30 μM FMRF-amide and 100 μM amiloride. Download figure Download PowerPoint Figure 2.Susceptibility of wild-type FaNaC and several mutants to 1 mM MTSET. Positive transfectants in the whole-cell clamp configuration were maintained at −40 mV. Bath and pipette solutions were of identical composition (140 mM NaCl, 10 mM HEPES, 1 mM CaCl2 pH 7.4). Cells were briefly perfused twice with the same solution containing 30 μM FMRF-amide to determine the maximal amplitude of the stimulation (Imax) before being rinsed until the baseline current was reached, and then perfused with 1 mM MTSET in the same solution for 3 min. The third peak corresponds to the channel stimulation by 30 μM FMRF-amide after 3 min of MTSET application followed by 1 min of rinse. In these conditions, the wild-type channel (WT) and mutants such as Ile75Cys are not affected by 1 mM MTSET, while mutants such as Val74Cys show a marked inhibition. Note that the Ser86Cys mutant, which exhibits a strong desensitization upon FMRF-amide repeated stimulations, regains activity and does not desensitize following 3 min of 1 mM MTSET exposure. F, 30 μM FMRF-amide; R, rinse. Download figure Download PowerPoint FaNaC does not contain any cysteine in its two transmembrane segments but 14 conserved cysteines are present in the extracellular loop of each monomer. As shown in Figure 2, the application of 1 mM MTSET, a cationic MTS, to the wild-type channel does not result in any significant modification of the channel activity. This indicates that these cysteines are not accessible, maybe because they form disulfide bridges, or that the covalent modification of accessible extracellular cysteines does not affect channel function. Accessibility of the positions of TM1 in the closed state of the channel We substituted individually the 23 amino acids of the FaNaC first transmembrane segment (positions 68–90) with cysteines using double-stranded site-directed mutagenesis (Quickchange Site-Directed Mutagenesis kit; Stratagene), directly on the pIRES-CD8 expression vector. All mutants were functional except the Trp70Cys mutant. This indicates an important role for this residue, which is confirmed by the fact that it is conserved in all the members of this family, such as the ASICs, MEC4, or the α subunit of the epithelial sodium channel (Figure 1A). The macroscopic electrophysiological properties (current–voltage relationship, FMRF-amide and amiloride sensitivities) of most of the mutants were essentially identical to those of the wild-type channel. It is worth noting that the Ser86Cys mutant, which was fully activated by FMRF-amide, rapidly desensitized with repetitive application of FMRF-amide (Figure 2). A similar observation was made for mutants Ala71Cys and Leu73Cys. Mutants such as Val74Cys cannot be fully activated by FMRF-amide following 3 min of MTSET application (Figure 2), indicating that this residue is accessible to the hydrophilic MTSET, and is therefore facing the channel pore. In contrast, Ile75Cys is unaffected by the same treatment (Figure 2), indicating that this position does not face the channel pore. All the mutants were tested under the same conditions. The compilation and statistical analysis of the results revealed two classes of mutants: (i) mutants that were significantly inhibited by MTSET (Student's t-test, p <0.001), with percentages of inhibition equal or superior to 50%; and (ii) mutants considered as not significant with a lower sensitivity to MTSET (<30%). The histogram presented in Figure 3 shows that positions 74, 76, 79, 83, 85, 86, 89 and 90 are facing the pore of the ion channel. To confirm these results, we determined the kinetic constants of inhibition of the various mutants by 1 mM MTSET using pulses of FMRF-amide. As shown in Table I, the results obtained are consistent with the percentages of inhibition determined after 3 min of MTSET exposure. Interestingly, the Ser86Cys mutant, which desensitized after FMRF-amide pulses, regained activity and did not desensitize following MTSET application (Figure 2). This results in a negative inhibition value (Figure 3) corresponding to an increase in channel activity caused by the MTSET application, and strongly suggests that this position is also accessible to MTSET. Figure 3.Histogram summarizing the sensitivities of the cysteine mutants of the FaNaC first transmembrane segment following 3 min of 1 mM MTSET application, and the corresponding SEMs. Experimental procedures were carried out as described in Figure 2 and in Materials and methods. Data for each mutant were obtained from at least six independent recordings and the percentages of inhibition calculated as (Imax − I)/Imax, where Imax is the current amplitude before 3 min of MTS application, and I the current amplitude following MTS application. Cysteine mutants at positions 74, 76, 79, 83, 85, 86, 89 and 90 are strongly affected by MTSET (Student's t -test, p <0.001), and the corresponding histogram bars are represented in dark grey. Download figure Download PowerPoint Table 1. Compilation of the kinetic constants of inhibition by 1 mM extracellular MTSET in the closed and open state Position Rate of inhibition in the closed state (mM−1 s −1) Rate of inhibition in the open state (mM−1 s−1) 89 0.23 ± 0.05 0.31 ± 0.042 85 0.352 ± 0.13 0.29 ± 0.018 84 0.04 ± 0.03 0.034 ± 0.007 79 0.423 ± 0.103 0.29 ± 0.06 77 0.036 ± 0.024 0.07 ± 0.015 76 0.325 ± 0.155 0.34 ± 0.064 74 0.36 ± 0.04 0.29 ± 0.057 72 0.15 ± 0.06 0.47 ± 0.09 Kinetic constants were determined as described in Materials and methods, using 1-min-spaced pulses of 30 μM FMRF-amide for determinations in the closed state and continuous FMRF-amide applications for determinations in the open state. Positions 84 and 77, which are not accessible to MTSET, are shown for comparison with the accessible positions. Note the concordance of the kinetic constants with the global percentages of inhibition shown in Figures 3 and 4, and the difference between the accessibility of position 72 in the closed and open state. The effect of MTSET, when applied intracellularly at 1 mM concentration in the patch pipette in whole-cell configuration, was tested from positions 68 to 74. As shown in Table II the rate constants determined for the cysteine mutants engineered at positions 68, 71, 72 and 74 are comparable to those of other unaffected positions such as 84 and 77 shown in Table I. Thus, we conclude that these positions are not accessible to intracellular MTSET in the closed conformation of the channel. In contrast, the Ile69Cys mutant exhibits a rate constant of inhibition one order of magnitude greater than those of the previously discussed mutants. This shows that position 69 is accessible to intracellular MTSET. Table 2. Compilation of the kinetic constants of inhibition by 1 mM intracellular MTSET in the closed and open state Position Rate of inhibition in the closed state (mM−1 s −1) Rate of inhibition in the open state (mM−1 s−1) 74 0.08 ± 0.055 0.09 ± 0.031 72 0.06 ± 0.075 0.17 ± 0.072 71 0.08 ± 0.03 0.36 ± 0.17 69 0.26 ± 0.037 0.12 ± 0.05 68 0.044 ± 0.011 0.099 ± 0.027 Kinetic constants were determined as described in Materials and methods, using 1-min-spaced pulses of 30 μM FMRF-amide for determinations in the closed state and continuous FMRF-amide application for determinations in the open state. Note the differences of accessibility between the closed and open states from position 69 to 72, showing that this region of the channel is involved in the gating mechanism. Accessibility of the positions of TM1 in the open state of the channel We tested the MTSET accessibility of various mutants from position 68 to 90 in the presence of FMRF-amide, i.e. in the open state of the channel. This set of experiments was made possible by the fact that, apart from Ala71Cys, Leu73Cys, Leu85Cys and Ser86Cys, all our MTSET-sensitive mutants did not significantly desensitize during continuous FMRF-amide application. Remarkably, position 72, which is affected only to a negligible extent in the closed state, becomes highly accessible in the open state (Figure 4A) when the MTSET is applied extracellularly. This result strongly suggests that the gating mechanism consists of conformational modifications resulting in the opening of a channel constriction located close to its intracellular side. To confirm this hypothesis, we tested the accessibility of positions 68–74 to intracellular MTSET in the open state of the channel. Using this procedure, we discovered that position 69, which is accessible to intracellular MTSET in the closed state of the channel, becomes slightly less sensitive to this reagent upon FMRF-amide application (Table II). Moreover, the cysteines introduced at positions 71 and 72, which were not accessible to intracellular MTSET in the closed state, become significantly affected in the open state. In contrast, position 74, which was not significantly affected by intracellular MTSET in the closed conformation, did not exhibit any detectable increase in its rate of inhibition in the open conformation. The simplest interpretation that can account for this apparently surprising observation is that a constriction situated in the vicinity of Leu72, which is unchanged in the Val74Cys mutant, blocks the accessibility of intracellular MTSET to the upper positions, even in the open state of the channel. Figure 4.(A) Open channel measurements for the Leu72Cys mutant. Positive transfectants in the whole-cell clamp configuration were maintained at −40 mV with the same bath and pipette solution (140 mM NaCl, 10 mM HEPES, 1 mM CaCl2 pH 7.4). Following one rapid stimulation with 30 μM FMRF-amide, the clamped cells were perfused for 3 min with 30 μM FMRF-amide plus or minus 1 mM MTSET. Note the stability of the FMRF-amide-evoked current, and its exponential decay in the presence of MTSET. F, 30 μM FMRF; R, rinse; M, 1 mM MTSET. The same protocol has been applied for all the mutants that were shown to be accessible to MTSET in the closed configuration, and the recordings are similar, apart from mutants Ala71Cys, Leu85Cys and Ser86Cys, which inactivated upon continuous FMRF-amide stimulation. (B) Histogram summarizing the sensitivity of the mutants to 1 mM MTSET in the presence and absence of FMRF-amide. Experimental procedures were carried out as described in (A), and the results were compiled from at least six independent experiments, as described in the legend to Figure 3 and in Materials and methods. Note that the percentages of inhibition are similar in the open and closed configuration, apart from position 72, which becomes unmasked upon channel opening. Download figure Download PowerPoint In summary, this set of results shows that the conformational changes leading to the opening of the channel take place in a region of the protein centred around Leu72. Location of the charge selectivity filter and the amiloride blockage site To gain information on the charge selectivity filter of the channel, we tested the inhibitory effect of the MTSES MTS on the TM1 cysteine mutants. MTSES is negatively charged and has a steric hindrance comparable to that of MTSET. No significant inhibition could be detected for positions 68–88 (Figure 5). In contrast, positions 89 and 90, which are located in the outer vestibule of the channel, are accessible to the anionic reagent. Therefore, FaNaC discriminates between cationic and anionic MTSs in a region situated in the pore entrance, between positions 85 and 89. Although other effects than accessibility could be responsible for these observations, these results suggest that the charge selectivity filter of the channel might be situated in the vestibule of the ion pore. Figure 5.Mapping of the charge selectivity filter and amiloride blockage positions. This histogram shows the accessibility of the MTSET-sensitive mutants to the anionic 10 mM MTSES in the closed configuration and the protection from 1 mM MTSET inhibition by 100 μM amiloride. For MTSES accessibility, the experimental procedure is the same as in Figure 2, with 10 mM MTSES replacing 1 mM MTSET. For amiloride protection, clamped cells were tested for FMRF-amide activation, perfused with a 100 μM amiloride solution for 3 min, rinsed, and the response to FMRF-amide was re-tested. This allowed us to verify that amiloride could be rinsed efficiently in the closed configuration. The amiloride solution was then switched after 30 s to 100 μM amiloride plus 1 mM MTSET for 3 min. The cells were then rinsed and tested for residual FMRF-amide activation. For comparison, 1 mM MTSET inhibition of the various mutants in the closed configuration is shown on the same graph. Download figure Download PowerPoint The diuretic amiloride is a classical inhibitor of ion channels of the ENaC/Degenerin family. Many data suggest that amiloride blocks these channels by occluding the ion pore (Palmer and Andersen, 1989). To gain access into the amiloride inhibition mechanism of FaNaC, we took advantage of our MTEST-accessible mutants to investigate whether amiloride could protect them from the covalent inhibitor. Continuous addition of amiloride on FaNaC, when activated by FMRF-amide, results in channel inactivation lasting after the inhibitor has been removed. Therefore, we investigated the potential protective effect of amiloride in the closed state of FaNaC. The Val74Cys, Ala76Cys and Thr79Cys MTSET-sensitive mutants are protected by amiloride, when added at a 100 μM concentration together with 1 mM MTSET (Figure 5). In contrast, the Leu83Cys to Arg90Cys MTSET-sensitive mutants are not protected in the same conditions. Amiloride may occlude the pore at a position located between Leu83 and Thr79, i.e. in the close vicinity of the conformational transition between the predicted α-helical and extended region of TM1. However, we cannot exclude the possibility that the binding of amiloride on a site situated apart from the ion pore may produce a conformational modification resulting in the narrowing of the channel near Thr79. Accessible cysteines in other members of the ENaC/Degenerin family Our results show that several residues of TM1 face the ion pore of FaNaC, which is constituted by a large aqueous cavity. To investigate whether these conclusions could be extended to other members of the family, we took advantage of the observation that ASIC1a and ASIC1b both have cysteines in their TM1 at positions corresponding to accessible positions in FaNaC (positions 83 and 85). As shown in Figure 6, these two channels are importantly affected by the MTSET treatment (2.5 mM for 5 min). Conversely, the rat ASIC2a channel, which does not possess any cysteine in its transmembrane region is practically insensitive to MTSET (2.5 mM for 5 min). When Val58 corresponding to Leu83 of FaNaC is mutated to cysteine (Figure 6, see inset), the mutant becomes significantly affected by MTSET. Taken together, the results obtained with the ASIC1a, 1b and 2a proteins suggest some similarity in the exposure of FaNaC and ASICs first transmembrane segment to pore lumen. However, slight structural differences may exist between these two classes of channels as illustrated by the ASIC2a Phe49Cys mutant. This position located deeper in the pore was not affected by the MTS reagent although the corresponding Val74Cys FaNaC mutant was highly sensitive to MTSET, suggesting that the interior of the ion pore is narrower in the proton-gated ion channels than in the FMRF-amide-activated channel. Figure 6.Effect of MTSET on wild-type and mutated ASICs. Oocytes injected with ASIC1a, ASIC1b and ASIC2a were exposed to MTSET (2.5 mM) for 5 min, and the percentages of inhibition of the H+-elicited currents were determined as described in Materials and methods. The ASIC1a and 1b isoforms possessing cysteines at positions corresponding to pore-facing residues of FaNaC (see inset) are significantly affected by the sulfhydryl reagent, whilst the ASIC2a isoform, which does not possess any cysteine in its first transmembrane segment is insensitive to MTSET. Introducing a cysteine at position 58 converts ASIC2a into a MTSET-sensitive isoform. However, the cysteine mutant introduced at position 49 (equivalent of position 74 in FaNaC) is not affected by MTSET. The number of oocytes tested in each condition is given above the histograms (mean ± SEM). Download figure Download PowerPoint Substitutions of key residues in TM1 We introduced several amino acid substitutions at three accessible positions situated, respectively, in the extracellular, middle and gating regions of TM1 (positions 89, 79 and 72). The mutated cDNAs were expressed in HEK293 cells and the effect of the introduced changes was evaluated in terms of function and ion selectivity of the mutated channels. The results obtained are shown in Table III. FaNaC TM1 is tolerant to substitutions that do not drastically change the nature of the residues at the above-mentioned positions. In contrast, more important mutations totally impair the functional expression of the channel. Remarkably, the Leu72Phe substitution results in a significant increase of the selectivity of the channel for sodium versus potassium (pNa+/pK+ = 4.96 ± 0.46 versus 2.84 ± 0.49 for the wild-type channel). This result confirms the importance of Leu72 in the channel function and gating. Table 3. Amino acid substitutions at key residues of FaNaC TM1 Residue Substitution pNa+/pK+ Wild type none 2.84 ± 0.49 Val89 arginine inactive lysine 2.14 ± 0.41 tyrosine 2.36 ± 0.54 Thr79 phenylalanine 2.67 ± 0.24 aspartic acid inactive Leu72 glycine 2.48 ± 0.24 phenylalanine 4.96 ± 0.46 tyrosine inactive aspartic acid inactive Various substitutions were introduced by site-directed mutagenesis, and the corresponding mutants were tested for their functional expression, and selectivity between sodium and potassium (pNa+/pK+). Note the presence of several inactive mutants for each tested position, and the change of relative permeability observed for the Leu72Phe mutant. Discussion This work is the first systematic exploration of the ion pore of a member of the ENaC/Degenerin gene family. In the closed state, nine positions of the first transmembrane segment of FaNaC are significantly accessible to the positively charged covalent reagent MTSET. This shows that a large part of the surface of this transmembrane segment is facing the lumen of the channel. This observation is surprising, due to the low conservation of TM1 in the ENaC/Degenerin family (Figure 1A), which led several authors to speculate that this transmembrane segment does not play a key role in ion translocation. However, domain swap experiments have suggested a possible implication of TM1 in the ion pore of the e