Produção Nacional
Revisado por pares
An Inhalation Chamber Model for Controlled Studies of Tobacco Smoke Toxicity in Rodents
2010; Elsevier BV; Volume: 46; Issue: 9 Linguagem: Inglês
10.1016/s1579-2129(10)70112-9
ISSN1885-6195
AutoresJosé Roberto Jardim, Luciana Bizeto, Anamaria Fleig Mayer, Aquiles Assunção Camelier, Fernanda Warken Rosa, Daniele Portela de Oliveira, Daiane Azevedo, Paulo Hilário Nascimento Saldiva, Mílton A. Martins, Jorge Bonassa, Oliver A. Nascimento,
Tópico(s)Smoking Behavior and Cessation
ResumoSmoking is a serious worldwide public health problem. Animal models act as a bridge between laboratory and human studies. The models applied are difficult to reproduce because of the use of different types of inhalation chambers and mainly because of the lack of continuous monitoring of smoke concentration.To develop an inhalation chamber for rats (with only the nose exposed) in which the amount of carbon monoxide (CO) can be maintained and monitored constantly.Male Wistar rats weighing 250g were exposed to 50ppm CO produced by the smoke from a filter-free cigarette. The animals were submitted to a single 2-h exposure and then sacrificed at 0, 4, 24 and 48h. The control group was left restrained inside the small perpendicular chambers, receiving only 5L/min of compressed air.The model was able to increase HbCO levels immediately after the end of exposure (p<0.001), with a decrease being observed from 2h onwards when compared to the levels of the control group. Plasma cotinine increased immediately after exposure, and showed still detectable levels at 2 and 4h (p<0.05).We conclude that the presented inhalation chamber system is able to maintain a controlled CO concentration in a model in which small animals are exposed to the inhalation of cigarette smoke, permitting well-controlled studies, as well as investigations involving other toxic gases and air pollutants.
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