On the mechanism of enzyme-catalysed gas—liquid reactions: Absorption of CO2, into buffer solutions containing carbonic anhydrase
1980; Elsevier BV; Volume: 35; Issue: 10 Linguagem: Inglês
10.1016/0009-2509(80)85039-1
ISSN1873-4405
AutoresE. Alper, Michael Lohse, W.‐D. Deckwer,
Tópico(s)Electrochemical sensors and biosensors
ResumoThe gas absorption with chemical reaction data of Lee and Tsao [1, 2] (i.e. oxidation of glucose solutions containing glucose oxidase or platinum deposited on activated carbon and absorption of carbondioxide into buffer solutions in the presence of carbonic anhydrase) were reexamined and various reasons for their apparent inconsistency with the classical gas absorption models were suggested. The rate of absorption of carbondioxide into 0.5 gmol/l Na2CO3 + 0.5 gmol/l NaHCO2 in the presence of carbonic anhydrase was measured in a stirred cell and also in a laminar jet and a wetted wall column aparatus. Enhancement in the absorption rate due to enzyme was observed in all three absorbers and the results were found to be compatible with classical mass transfer models (such as Higbie's and Danckwerts' models). On the contrary to Tsao[2], who proposed a two-zone model, which assumes a surface-excess of the enzyme at the gas—liquid interface, these results suggest that the carbonic anhydrase distribution in the liquid is probably uniform.
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