Produção Nacional
Revisado por pares
DOES INTRATESTICULAR TESTOSTERONE ADMINISTRATION MODIFY THE EVOLUTION OF TRANSITORY TESTICULAR ISCHEMIA IN PREPUBERTAL RATS?
1998; Lippincott Williams & Wilkins; Volume: 159; Issue: 5 Linguagem: Inglês
10.1097/00005392-199805000-00106
ISSN1527-3792
AutoresCarlos Teodósio Da Ros, Cláudio Telöken, M. Tannhauser, A Hartmann,
Tópico(s)Sexual Differentiation and Disorders
ResumoNo AccessJournal of UrologyInvestigative Urology1 May 1998DOES INTRATESTICULAR TESTOSTERONE ADMINISTRATION MODIFY THE EVOLUTION OF TRANSITORY TESTICULAR ISCHEMIA IN PREPUBERTAL RATS? CARLOS TEODOSIO DA ROS, CLAUDIO TELOKEN, MARIO TANNHAUSER, and ANTONIO HARTMANN CARLOS TEODOSIO DA ROSCARLOS TEODOSIO DA ROS , CLAUDIO TELOKENCLAUDIO TELOKEN , MARIO TANNHAUSERMARIO TANNHAUSER , and ANTONIO HARTMANNANTONIO HARTMANN View All Author Informationhttps://doi.org/10.1097/00005392-199805000-00106AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: Testicular torsion followed by ischemia results in variable degrees of infertility and until now there appears to be no effective way to recover it. Testosterone participation in the maintenance of male sexual organs and spermatogenesis led us to hypothesize that intratesticular administration could recover ischemic injury. Materials and Methods: We divided 40 Wistar rats in 2 groups, of 20 each. One group was control and the other underwent a 120-minute testicular ischemia by means of a vascular clamp on the left spermatic cord. Each group was further subdivided in 2 subgroups. The first one was observed and the second received intratesticular testosterone 25 mg. starting on the third day after injury and during the next 7 consecutive days. Half the animals were sacrificed 30 days after injury and the remaining ones after 60 days. Weight, volume, number of seminiferous tubules, histology and spermatogenesis of the same side and contralateral testes were examined. For statistical analysis ANOVA and Fisher's tests were applied. Results: It was found that testosterone was capable of acting upon volume and weight of the left testis (p = 0.0001). The animals receiving intratesticular testosterone showed lower testicular weight and volume after 30 and 60 days, respectively. This subgroup also showed a higher number of seminiferous tubules, modified histology and absent spermatogenesis suggesting testicular atrophy. Conclusions: We concluded that intratesticular injection of testosterone 25 mg. once a day during 7 consecutive days after transitory testicular ischemia causes ipsilateral testis atrophy. The animals in control group showed testicular histological recovery 60 days after injury. There were no significant histological differences in the contralateral testes. References 1 : Testicular size: the effects of aging, malnutrition and illness. J. Androl.1985; 6: 144. Google Scholar 2 : Testicular size in infertile men: relationship to semen characteristics and hormonal blood levels. Br. J. Urol.1989; 64: 632. Google Scholar 3 : Sex hormones. In: Martindale-The Extra Pharmacopoeia.. London: The Pharmaceutical Press1993: 1166. Google Scholar 4 : Restoration of spermatogenesis and subsequent fertility by direct intratesticular hormonal therapy. J. 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Google Scholar 17 : Myoid cell proliferation in rat seminiferous tubules after ischaemic testicular atrophy induced by epinephrine. Morphometric and immunohistochemical (bromo-deoxyuridine and PCNA) studies. Int. J. Androl.1995; 18: 13. Google Scholar 18 : Variability of ischemia during spermatic cord torsion in the rat. J. Urol.1994; 151: 1070. Abstract, Google Scholar 19 : Endocrine and exocrine responses to testicular torsion in prepubertal and adult rats. J. Urol.1995; 153: 427A. Google Scholar From the Department of Pharmacology and Toxicology and Pathology of the Federal Faculty of Medicine, Porto Alegre, Brazil.© 1998 by American Urological Association, Inc.FiguresReferencesRelatedDetails Volume 159 Issue 5 May 1998 Page: 1752-1754 Advertisement Copyright & Permissions© 1998 by American Urological Association, Inc.Metrics Author Information CARLOS TEODOSIO DA ROS More articles by this author CLAUDIO TELOKEN More articles by this author MARIO TANNHAUSER More articles by this author ANTONIO HARTMANN More articles by this author Expand All Advertisement PDF downloadLoading ...
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