Produção Nacional
Revisado por pares
Pharmacological Modulation of the Late Eosinophilia Induced by Antigen in Actively Sensitized Rats
1992; Karger Publishers; Volume: 98; Issue: 4 Linguagem: Inglês
10.1159/000236211
ISSN1423-0097
AutoresPatrı́cia Silva, Marco A. Martins, Márcia C.R. Lima, Alessandra Castro Alves, Bruno L. Diaz, Renato S.B. Cordeiro,
Tópico(s)Exercise and Physiological Responses
ResumoThe intrathoracic injection of ovalbumin (12 μg/cavity) into actively sensitized rats led to a long-lasting eosinophil recruitment, which appeared 24 h after stimulation. In this study, pharmacological antagonists were used in order to evaluate the potential involvement of arachidonic acid metabolites and PAF-acether in the pleural eosinophil accumulation by antigen. Administration of the cyclooxygenase inhibitor indomethacin (2 mg/kg, i.p.), 1 h before the antigen challenge, failed to modify the 24-hour eosinophilia. In contrast, the dual cyclooxygenase and lipoxygenase inhibitor BW 755C and the more selective inhibitor BW A4C (5 and 10 μg/cavity, i.t.), injected 1 h before the antigen, were effective. Similarly, the PAF-acether antagonists BN 52021 and WEB 2086 (20 mg/kg, i.p.) abrogated the eosinophil accumulation, which was also sensitive to the topical treatment with the glucocorticoid dexamethasone (5 and 10 μg/ cavity). Our findings suggest that the antigen-induced eosinophil mobilization is dependent on lipoxygenase derivatives and PAF-acether, but not on prostaglandins.
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