DNA Origami Design of Dolphin-Shaped Structures with Flexible Tails
2008; American Chemical Society; Volume: 2; Issue: 6 Linguagem: Inglês
10.1021/nn800215j
ISSN1936-086X
AutoresEbbe Sloth Andersen, Mingdong Dong, Morten Muhlig Nielsen, Kasper Jahn, Allan Lind-Thomsen, Wael Mamdouh, Kurt V. Gothelf, Flemming Besenbacher, Jørgen Kjems,
Tópico(s)Bacteriophages and microbial interactions
ResumoThe DNA origami method allows the folding of long, single-stranded DNA sequences into arbitrary two-dimensional structures by a set of designed oligonucleotides. The method has revealed an unexpected strength and efficiency for programmed self-assembly of molecular nanostructures and makes it possible to produce fully addressable nanostructures with wide-reaching application potential within the emerging area of nanoscience. Here we present a user-friendly software package for designing DNA origami structures (http://www.cdna.dk/origami) and demonstrate its use by the design of a dolphin-like DNA origami structure that was imaged by high-resolution AFM in liquid. The software package provides automatic generation of DNA origami structures, manual editing, interactive overviews, atomic models, tracks the design history, and has a fully extendable toolbox. From the AFM images, it was demonstrated that different designs of the dolphin tail region provided various levels of flexibility in a predictable fashion. Finally, we show that the addition of specific attachment sites promotes dimerization between two independently self-assembled dolphin structures, and that these interactions stabilize the flexible tail.
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