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Quantitative Matrix-Assisted Laser Desorption Ionization−Fourier Transform Ion Cyclotron Resonance (MALDI−FT-ICR) Peptide Profiling and Identification of Multiple-Sclerosis-Related Proteins

2009; American Chemical Society; Volume: 8; Issue: 3 Linguagem: Inglês

10.1021/pr8010155

1535-3907

Marcel P. Stoop, Lennard J. M. Dekker, Mark K. Titulaer, Robert‐Jan A. N. Lamers, Peter C. Burgers, Peter A.E. Sillevis Smitt, Alain J. van Gool, Theo M. Luider, Rogier Q. Hintzen,

Molecular Biology Techniques and Applications

We introduce a matrix-assisted laser desorption ionization−Fourier transform ion cyclotron resonance (MALDI−FT-ICR) method for quantitative peptide profiling, using peak height as a measure for abundance. Relative standard deviations in peak height of peptides spiked over 3 orders of magnitude in concentration were below 10% and allowed for accurate comparisons between multiple sclerosis and controls. Application on a set of 163 cerebrospinal fluid (CSF) samples showed significantly differential abundant peptides, which were subsequently identified into proteins (e.g., chromogranin A, clusterin, and complement C3).