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Intraocular microdialysis with permanently implanted probes in rabbit

1991; Wiley; Volume: 69; Issue: 5 Linguagem: Inglês

10.1111/j.1755-3768.1991.tb04849.x

1755-3768

Janina Waga, Akiko Ohta, B. Ehinger,

Veterinary Pharmacology and Anesthesia

Abstract The technique of microdialysis in vivo, much used in brain experiments, has been adapted for intraocular use. A new probe was designed, made from a soft tube with the dialysis membrane mounted in a fenestrated protecting sleeve, facing one side. A special surgical procedure was developed for the long‐term implantation of the probes. They stay functional in the eyes for several weeks or more. Previously published intraocular microdialysis studies were short‐term experiments. The acute surgical trauma is likely to affect the concentration of many compounds in the vitreous, and this effect is minimized with long‐term probes.