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Control of mitochondrial matrix calcium: Studies using fluo-3 as a fluorescent calcium indicator

1990; Elsevier BV; Volume: 167; Issue: 1 Linguagem: Inglês

10.1016/0006-291x(90)91743-c

1090-2104

Alfredo Saavedra‐Molina, Salvador Uribe‐Carvajal, Thomas M. Devlin,

Peroxisome Proliferator-Activated Receptors

Fluo-3, a fluorescent Ca2+ indicator, is sequestered by isolated rat liver mitochondria and is an effective probe for evaluating the concentration and kinetics of change of mitochondrial matrix ionized calcium ([Ca2+]m) under a variety of conditions. At the wavelengths employed, there is no significant interference by auto-fluorescence. There is an insignificant release of the indicator over four hours and the loading and presence of fluo-3 has no effect on respiratory rate or oxidative phosphorylation. The [Ca2+]m steady state can be altered by the assay conditions, i.e. the presence of extramitochondrial Ca2+, Mg2+, phosphate and respiratory inhibitors. The total matrix ionized calcium represents a small percent (<0.01%) of the total mitochondrial calcium.