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Human endothelial colony-forming cells serve as trophic mediators for mesenchymal stem cell engraftment via paracrine signaling

2014; National Academy of Sciences; Volume: 111; Issue: 28 Linguagem: Inglês

10.1073/pnas.1405388111

1091-6490

Ruei‐Zeng Lin, Rafael Moreno‐Luna, Dan Li, Shou-Ching Jaminet, Arin K. Greene, Juan M. Melero‐Martin,

Congenital heart defects research

Significance Endothelial colony-forming cells (ECFCs) circulate in peripheral blood and contribute to the formation of new vasculature. Here, we demonstrate that ECFCs can also function as paracrine mediators prior to the establishment of blood perfusion, modulating the regenerative potential of human mesenchymal stem cells (MSCs). We show that MSCs are highly dependable on platelet-derived growth factor BB and that ECFCs provide critical angiocrine factors needed to preserve MSCs as viable. This early angiocrine support ultimately enables extensive engraftment and long-term differentiation of transplanted MSCs. This study provides new insights into the biological attributes of ECFCs that may expand their therapeutic potential. We foresee the use of ECFCs as a means to improve the outcome of MSC transplantation.